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Literature summary for 1.7.3.6 extracted from

  • Wehrfritz, J.M.; Reilly, A.; Spiro, S.; Richardson, D.J.
    Purification of hydroxylamine oxidase from Thiosphaera pantotropha. Identification of electron acceptors that couple heterotrophic nitrification to aerobic denitrification (1993), FEBS Lett., 335, 246-250.
    View publication on PubMed
Search for more literature for 1.7.3.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 ferricytochrome c551 pH 7.8, 25°C Paracoccus denitrificans
0.033
-
 pseudoazurin pH 7.8, 25°C Paracoccus denitrificans

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Paracoccus denitrificans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe non-heme iron enzyme, the enzyme could possess a non-heme ferric iron centre that is lost when the 20000 Da polypeptide is subjected to SDS-PAGE, but that can be reconstituted by addition of Fe3+ ions to the refolded enzyme Paracoccus denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 x * 20000, SDS-PAGE Paracoccus denitrificans

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
 i.e. Thiosphaera pantotropha, heterotrophic nitrifying bacterium
-

Purification (Commentary)

Purification (Comment) Organism
partial Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + ferricytochrome c551
-
 Paracoccus denitrificans nitroxyl + ferrocytochrome c551 + H+
-
 ?
hydroxylamine + pseudoazurin
-
 Paracoccus denitrificans nitrite + reduced pseudoazurin
-
 ?

Subunits

Subunits Comment Organism
? x * 20000, SDS-PAGE Paracoccus denitrificans

Cofactor

Cofactor Comment Organism Structure
additional information non-heme iron enzyme Paracoccus denitrificans