BRENDA - Enzyme Database
show all sequences of 1.7.3.6

The biochemical characterization of a novel non-heme-iron hydroxylamine oxidase from Paracoccus denitrificans GB17

Moir, J.W.B.; Wehrfritz, J.M.; Spiro, S.; Richardson, D.J.; Biochem. J. 319, 823-827 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
the cyanide-sensitive form of the enzyme exists only during turnover
Paracoccus denitrificans
EDTA
1 mM, in 10 mM Tris/HCl, pH 8.0, almost complete inhibition
Paracoccus denitrificans
NaCl
15 mM, 50% inhibition of activity with horse heart cytochrome, activity with pseudoazurin or cytochrome c550 is inhibited only above 100 mM
Paracoccus denitrificans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
contains 3-5 nonhaem, non-iron-sulfur iron atoms as prosthetic groups, predominantly coordinated by carboxylate ligands
Paracoccus denitrificans
Organism
Organism
UniProt
Commentary
Textmining
Paracoccus denitrificans
-
strain GB17, heterotrophic nitrifier
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
hydroxylamine + ferricytochrome c550
under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction
394342
Paracoccus denitrificans
nitroxyl + ferrocytochrome c550 + H+
-
-
-
?
hydroxylamine + O2
horse heart cytochrome. Under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction
394342
Paracoccus denitrificans
nitrite + H2O + H+
-
-
-
?
hydroxylamine + pseudoazurin
under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction
394342
Paracoccus denitrificans
nitrite + reduced pseudoazurin
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
hydroxylamine oxidase
-
Paracoccus denitrificans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.53
-
hydroxylamine
pH 8, temperature not specified in the publication
Paracoccus denitrificans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
activity with horse heart cytochrome c
Paracoccus denitrificans
10
-
activity with pseudoazurin
Paracoccus denitrificans
11
-
activity with cytochrome c550 continues to increase to pH 11
Paracoccus denitrificans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.3
11.5
pH 7.3: about 35% of maximal activity, pH 11.5: about 50% of maximal activity activity with pseudoazurin
Paracoccus denitrificans
7.6
10
pH 7.6: about 50% of maximal activity, pH 10.0: about 35% of maximal activity with horse heart cytochrome c
Paracoccus denitrificans
8
11
activity with cytochrome c550 continues to increase to pH 11, activity at pH 8 is about 50% of the activity at pH 11
Paracoccus denitrificans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
the cyanide-sensitive form of the enzyme exists only during turnover
Paracoccus denitrificans
EDTA
1 mM, in 10 mM Tris/HCl, pH 8.0, almost complete inhibition
Paracoccus denitrificans
NaCl
15 mM, 50% inhibition of activity with horse heart cytochrome, activity with pseudoazurin or cytochrome c550 is inhibited only above 100 mM
Paracoccus denitrificans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
contains 3-5 nonhaem, non-iron-sulfur iron atoms as prosthetic groups, predominantly coordinated by carboxylate ligands
Paracoccus denitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
hydroxylamine + ferricytochrome c550
under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction
394342
Paracoccus denitrificans
nitroxyl + ferrocytochrome c550 + H+
-
-
-
?
hydroxylamine + O2
horse heart cytochrome. Under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction
394342
Paracoccus denitrificans
nitrite + H2O + H+
-
-
-
?
hydroxylamine + pseudoazurin
under aerobic assay conditions nitrite is the major reaction product but N2O is also detected at low levels. Under anaerobic conditions no nitrite is produced but N2O is detected, though at non-stoichiometric levels. The production of nitrite by Paracoccus denitrificans hydroxylamine oxidase is an oxygen-dependent reaction
394342
Paracoccus denitrificans
nitrite + reduced pseudoazurin
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.53
-
hydroxylamine
pH 8, temperature not specified in the publication
Paracoccus denitrificans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
activity with horse heart cytochrome c
Paracoccus denitrificans
10
-
activity with pseudoazurin
Paracoccus denitrificans
11
-
activity with cytochrome c550 continues to increase to pH 11
Paracoccus denitrificans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.3
11.5
pH 7.3: about 35% of maximal activity, pH 11.5: about 50% of maximal activity activity with pseudoazurin
Paracoccus denitrificans
7.6
10
pH 7.6: about 50% of maximal activity, pH 10.0: about 35% of maximal activity with horse heart cytochrome c
Paracoccus denitrificans
8
11
activity with cytochrome c550 continues to increase to pH 11, activity at pH 8 is about 50% of the activity at pH 11
Paracoccus denitrificans
Other publictions for EC 1.7.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741887
Cedervall
Structural studies of hydroxy ...
Nitrosomonas europaea
Biochemistry
52
6211-6218
2013
-
-
-
1
-
-
-
-
1
-
-
2
-
4
-
-
1
-
-
-
-
-
2
1
3
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
1
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
394342
Moir
The biochemical characterizati ...
Paracoccus denitrificans
Biochem. J.
319
823-827
1996
-
-
-
-
-
-
3
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
3
3
-
-
-
-
-
-
-
-
721442
Wehrfritz
Hydroxylamine oxidation in het ...
Pseudomonas sp., Pseudomonas sp. S2.14
Arch. Microbiol.
166
421-424
1996
-
-
-
-
-
-
-
2
1
-
1
-
-
9
-
-
-
-
-
-
1
-
4
1
2
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
394345
Wehrfritz
Purification of hydroxylamine ...
Paracoccus denitrificans
FEBS Lett.
335
246-250
1993
-
-
-
-
-
-
-
2
1
1
1
-
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
1
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723384
Kurokawa
-
A hydroxylamine-cytochrome c r ...
Arthrobacter globiformis, Arthrobacter globiformis IFO 3062
Plant Cell Physiol.
26
1439-1442
1985
-
-
-
-
-
-
2
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
1
-
-
1
-
-
1
-
-
-
1
-
-
-
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
394328
Hooper
Hydroxylamine oxidoreductase f ...
Nitrosomonas europaea
Biochemistry
16
455-459
1977
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-