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Literature summary for 1.7.3.5 extracted from
- Propionate-3-nitronate oxidase from Penicillium atrovenetum is a flavoprotein which initiates the autoxidation of its substrate by O2 (1987), J. Biol. Chem., 262, 14428-14434.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| propane-2-nitronate | increases the enzyme activity over 40fold when added to a reaction mixture that contains propionate-3-nitronate, the stimulation can be partially inhibited by superoxide dismutase | Penicillium atrovenetum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Penicillium atrovenetum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE in presence of mercaptoethanol | Penicillium atrovenetum |
| 73000 | - |
gel filtration | Penicillium atrovenetum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium atrovenetum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Penicillium atrovenetum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3-aci-nitropropanoate + O2 + H2O = 3-oxopropanoate + nitrite + H2O2 | primary products of the enzymatic reaction probably is the nitropropanoate free radical and superoxide | Penicillium atrovenetum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Penicillium atrovenetum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160 | - |
butyrate-4-nitronate as substrate | Penicillium atrovenetum |
| 274 | - |
propionate-3-nitronate as substrate | Penicillium atrovenetum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-aci-nitropropanoate + O2 | - |
Penicillium atrovenetum | 3-oxopropanoate + nitrite + H2O2 | - |
? | |
| butyrate-4-nitronate + O2 | - |
Penicillium atrovenetum | succinate + nitrite + H2O2 | - |
? | |
| additional information | neither cytochrome c nor K3Fe(CN)6 serve as oxidants, other substrates tested are oxidized less than 2% the rate of 3-nitropropanoate | Penicillium atrovenetum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE in presence of mercaptoethanol | Penicillium atrovenetum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Penicillium atrovenetum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 350 | - |
3-aci-Nitropropanoate | - |
Penicillium atrovenetum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at | Penicillium atrovenetum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | flavoprotein | Penicillium atrovenetum | |
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