General Stability | Organism |
---|---|
uricase is reversibly inactivated in solutions of low ionic strength (like during dialysis against water). After incubation for 2 h in 100 mM sodium chloride in water at 4°C, the dialysis-inactivated uricase shows about 70% of the maximal specific activity. After incubation for 2 h in 100 mM Tris-HCl pH 8.0 plus 100 mM NaCl at 4°C, the dialysis-inactivated uricase shows about 90% of the maximal specific activity. After pre-incubation for 0.5 h in sodim borate plus 100 mM NACL at 25°C followed by the direct addition of urate to measure its activity, the dialysis-inactivated uricase shows about 80% of the maximal specific activity | Metabacillus fastidiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
144000 | - |
native enzyme, gel filtration | Metabacillus fastidiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metabacillus fastidiosus | C5HDG5 | - |
- |
Metabacillus fastidiosus ATCC 26904 | C5HDG5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Metabacillus fastidiosus | 5-hydroxyisourate + H2O2 | - |
? | |
urate + O2 + H2O | - |
Metabacillus fastidiosus ATCC 26904 | 5-hydroxyisourate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 000, SDS-PAGE, homotetrameric uricase in water dissociates into inactive homodimers that can form active homotetramers again in solutions of high ionic strength | Metabacillus fastidiosus |
Synonyms | Comment | Organism |
---|---|---|
uricase | - |
Metabacillus fastidiosus |