Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.3.3 extracted from

  • Imhoff, R.D.; Power, N.P.; Borrok, M.J.; Tipton, P.A.
    General base catalysis in the urate oxidase reaction: evidence for a novel Thr-Lys catalytic diad (2003), Biochemistry, 42, 4094-4100.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D79A the KM-value for urate is 82% of the wild-type value Bacillus subtilis
K9M mutant enzyme has a maximal velocity of 0.4% of the wild-type value. Ionization at pH 6.4 that is observed with the wild-type enzyme is absent in the mutant. The KM-value for urate is 1.5fold higher than that of the wild-type enzyme Bacillus subtilis
T69A mutant enzyme has a maximal velocity of 3% of the wild-type value. Ionization at pH 6.4 that is observed with the wild-type enzyme is absent in the mutant. The KM-value for urate is 5fold higher than that of the wild-type enzyme Bacillus subtilis
T69A/K9M the KM-value for urate is 16.1fold higher than that of the wild-type enzyme Bacillus subtilis
T69V the KM-value for urate is 30.9fold higher than that of the wild-type enzyme Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
Urate pH 8.0, mutant enzyme D70A Bacillus subtilis
0.034
-
Urate pH 8.0, wild-type enzyme Bacillus subtilis
0.051
-
Urate pH 8.0, mutant enzyme K9M Bacillus subtilis
0.17
-
Urate pH 8.0, mutant enzyme T69A Bacillus subtilis
0.547
-
Urate pH 8.0, mutant enzyme T69A/K9M Bacillus subtilis
1.05
-
Urate pH 8.0, mutant enzyme T69V Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + O2 + H2O it is proposed that T69 and K9 form a catalytic diad in which K9 deprotonates T69 to allow it to abstract the proton from the N9 position of the substrate to generate the dianion Bacillus subtilis 5-hydroxyisourate + H2O2
-
?