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Literature summary for 1.7.3.3 extracted from
- Uricase from soybean root nodules: purification, properties, and comparison with the enzyme from cowpea (1983), Arch. Biochem. Biophys., 226, 190-197.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenine | weak | Glycine max |  |
| allantoin | weak | Glycine max | |
| glutamine | weak | Glycine max | |
| NH4+ | weak | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
uric acid | - |
Glycine max | |
| 0.031 | - |
O2 | - |
Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | 35000 | SDS-PAGE, gel filtration | Glycine max |
| 68000 | - |
gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Glycine max | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.85 | - |
- |
Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Glycine max | 5-hydroxyisourate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | at pH 8.8 the enzyme associates to form a dimer of about 68000 | Glycine max |
| monomer | native enzyme at pH 7.5 is a monomer with MW 32000-35000 | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Glycine max |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10.2 | pH 8.0: about 35% of activity maximum, pH 10.2: about 55% of activity maximum | Glycine max |
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Release 2023.1 (January 2023)
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