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Literature summary for 1.7.2.9 extracted from

  • Versantvoort, W.; Pol, A.; Jetten, M.; van Niftrik, L.; Reimann, J.; Kartal, B.; Op den Camp, H.
    Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs (2020), Proc. Natl. Acad. Sci. USA, 117, 24459-24463 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
hydroxylamine pH 7.5, 60┬░C Methylacidiphilum fumariolicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
250000
-
and 500000, native PAGE Methylacidiphilum fumariolicum
500000
-
and 250000, native PAGE Methylacidiphilum fumariolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxylamine + 3 ferricytochrome c Methylacidiphilum fumariolicum
-
nitric oxide + 3 ferrocytochrome c + 3 H+
-
?
hydroxylamine + 3 ferricytochrome c Methylacidiphilum fumariolicum SolV
-
nitric oxide + 3 ferrocytochrome c + 3 H+
-
?

Organism

Organism UniProt Comment Textmining
Methylacidiphilum fumariolicum
-
-
-
Methylacidiphilum fumariolicum SolV
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + 3 ferricytochrome c
-
Methylacidiphilum fumariolicum nitric oxide + 3 ferrocytochrome c + 3 H+
-
?
hydroxylamine + 3 ferricytochrome c
-
Methylacidiphilum fumariolicum SolV nitric oxide + 3 ferrocytochrome c + 3 H+
-
?

Subunits

Subunits Comment Organism
? x * 83860, calculated from sequence Methylacidiphilum fumariolicum

Synonyms

Synonyms Comment Organism
haoA
-
Methylacidiphilum fumariolicum
Mfumv2_2472
-
Methylacidiphilum fumariolicum
mHAO
-
Methylacidiphilum fumariolicum

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
60
-
-
Methylacidiphilum fumariolicum

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
80
-
active up to Methylacidiphilum fumariolicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.2
-
hydroxylamine pH 7.5, 60┬░C Methylacidiphilum fumariolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Methylacidiphilum fumariolicum

Cofactor

Cofactor Comment Organism Structure
heme 9 heme c moieties per subunit Methylacidiphilum fumariolicum

General Information

General Information Comment Organism
physiological function in methanotrophs, mHAO efficiently removes hydroxylamine, which severely inhibits calcium-dependent and lanthanide-dependent methanol dehydrogenases Methylacidiphilum fumariolicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5100
-
hydroxylamine pH 7.5, 60┬░C Methylacidiphilum fumariolicum