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Literature summary for 1.7.2.8 extracted from

  • Shimamura, M.; Nishiyama, T.; Shinya, K.; Kawahara, Y.; Furukawa, K.; Fujii, T.
    Another multiheme protein, hydroxylamine oxidoreductase, abundantly produced in an anammox bacterium besides the hydrazine-oxidizing enzyme (2008), J. Biosci. Bioeng., 105, 243-248.
    View publication on PubMed
Search for more literature for 1.7.2.8

Cloned(Commentary)

Cloned (Comment) Organism
hao gene, indicating DNA and amino acid sequence determination and analysis Candidatus Jettenia caeni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 hydrazine pH 8.0, 35°C Candidatus Jettenia caeni
0.033
-
 hydroxylamine pH 8.0, 35°C Candidatus Jettenia caeni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 2 * 53000, SDS-PAGE Candidatus Jettenia caeni
118000
-
 gel filtration Candidatus Jettenia caeni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Candidatus Jettenia caeni HAO is an indispensable enzyme and well conserved in anammox bacteria ?
-
 ?

Organism

Organism UniProt Comment Textmining
Candidatus Jettenia caeni
-
-
-
Candidatus Jettenia caeni A9ZRZ6 gene hao
-

Purification (Commentary)

Purification (Comment) Organism
hydroxylamine oxidoreductase (HAO) is purified from anammox sludge in which an anammox bacterium, strain KSU-1, is dominant Candidatus Jettenia caeni
native enzyme 15fold by anion exchange chromatography and gel filtration Candidatus Jettenia caeni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.6
-
-
 Candidatus Jettenia caeni
9.6
-
 partially purified native enzyme Candidatus Jettenia caeni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydrazine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide via phenazine methosulfate Candidatus Jettenia caeni ? + reduced methylthiazolyltetrazolium bromide
-
 ?
hydrazine + NH3 + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
-
 Candidatus Jettenia caeni ?
-
 ?
hydrazine + NH3 + phenazine methosulfate
-
 Candidatus Jettenia caeni ?
-
 ?
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate Candidatus Jettenia caeni ? + reduced 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
-
 ?
hydroxylamine + NH3 + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
-
 Candidatus Jettenia caeni ?
-
 ?
hydroxylamine + NH3 + phenazine methosulfate
-
 Candidatus Jettenia caeni NO2 + H2O + reduced phenazine methosulfate
-
 ?
additional information HAO is an indispensable enzyme and well conserved in anammox bacteria Candidatus Jettenia caeni ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Candidatus Jettenia caeni

Synonyms

Synonyms Comment Organism
HAO
-
 Candidatus Jettenia caeni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Candidatus Jettenia caeni
65
-
-
 Candidatus Jettenia caeni

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 8.0, 2 min, stable below Candidatus Jettenia caeni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Candidatus Jettenia caeni
8 8.5
-
 Candidatus Jettenia caeni

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 35°C, 20 min, stable Candidatus Jettenia caeni

Cofactor

Cofactor Comment Organism Structure
cytochrome P468 chromophore Candidatus Jettenia caeni
cytochrome P468 the enzyme has a cytochrome P468 chromophore Candidatus Jettenia caeni
heme c-heme content is 16.1 per 18000 Da protein Candidatus Jettenia caeni
heme the hao gene indicats eight c-type heme binding motifs and is a multiheme protein containing covalently bound c-type heme Candidatus Jettenia caeni
additional information the sequence deduced from the hao gene indicates eight c-type heme binding motifs Candidatus Jettenia caeni
phenazine methosulfate
-
 Candidatus Jettenia caeni