Cloned (Comment) | Organism |
---|---|
expressed in Wolinella succinogenes as enzyme-maltose binding protein fusion | Campylobacter fetus |
expressed in Wolinella succinogenes as enzyme-maltose binding protein fusion | Caminibacter mediatlanticus |
expressed in Wolinella succinogenes as enzyme-maltose binding protein fusion | Nautilia profundicola |
expressed in Wolinella succinogenes as enzyme-maltose binding protein fusion | Campylobacter curvus |
expression in Wolinella succinogenes as maltose binding protein fusion | Campylobacter fetus |
expression in Wolinella succinogenes as maltose binding protein fusion | Caminibacter mediatlanticus |
expression in Wolinella succinogenes as maltose binding protein fusion | Nautilia profundicola |
expression in Wolinella succinogenes as maltose binding protein fusion | Campylobacter curvus |
Protein Variants | Comment | Organism |
---|---|---|
W428Y | mutant contains a tyrosine residue at the position corresponding to that in Nitrosomonas europaea Hao, where it is described to crosslink the monomers of the Hao trimer. Hydoxylamine reductase activity is similar to wild-type, nitrite reductase activity decreases considerably | Campylobacter curvus |
W428Y | the mutant produces a higher amount of nitrite from hydroxylamine oxidation than the corresponding wild type enzyme | Campylobacter curvus |
W434Y | mutant contains a tyrosine residue at the position corresponding to that in Nitrosomonas europaea Hao, where it is described to crosslink the monomers of the Hao trimer. Hydoxylamine reductase activity is similar to wild-type, nitrite reductase activity decreases considerably | Campylobacter fetus |
W464Y | mutant contains a tyrosine residue at the position corresponding to that in Nitrosomonas europaea Hao, where it is described to crosslink the monomers of the Hao trimer. Hydoxylamine reductase activity is similar to wild-type, nitrite reductase activity increases 3fold | Caminibacter mediatlanticus |
W464Y | the mutant produces a higher amount of nitrite from hydroxylamine oxidation than the corresponding wild type enzyme | Caminibacter mediatlanticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
nitrite | wild-type, pH 7.0, 37°C | Campylobacter fetus | |
1.5 | - |
nitrite | wild-type, pH 7.0, 37°C | Caminibacter mediatlanticus | |
1.9 | - |
hydroxylamine | wild-type, pH 7.0, 37°C | Campylobacter fetus | |
2.39 | - |
nitrite | mutant W434Y, pH 7.0, 37°C | Campylobacter fetus | |
2.7 | - |
nitrite | wild-type, pH 7.0, 37°C | Campylobacter curvus | |
2.99 | - |
nitrite | mutant W428Y, pH 7.0, 37°C | Campylobacter curvus | |
4.6 | - |
nitrite | mutant W464Y, pH 7.0, 37°C | Caminibacter mediatlanticus | |
6 | - |
hydroxylamine | wild-type, pH 7.0, 37°C | Caminibacter mediatlanticus | |
6.6 | - |
hydroxylamine | wild-type, pH 7.0, 37°C | Campylobacter curvus | |
6.75 | - |
hydroxylamine | mutant W428Y, pH 7.0, 37°C | Campylobacter curvus | |
7.4 | - |
hydroxylamine | mutant W434Y, pH 7.0, 37°C | Campylobacter fetus | |
16.9 | - |
hydroxylamine | mutant W464Y, pH 7.0, 37°C | Caminibacter mediatlanticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + H2O + 4 ferricytochrome c | Campylobacter fetus | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
hydroxylamine + H2O + 4 ferricytochrome c | Caminibacter mediatlanticus | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
hydroxylamine + H2O + 4 ferricytochrome c | Nautilia profundicola | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
hydroxylamine + H2O + 4 ferricytochrome c | Campylobacter curvus | - |
nitrite + 4 ferrocytochrome c + 5 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caminibacter mediatlanticus | - |
- |
- |
Campylobacter curvus | - |
- |
- |
Campylobacter fetus | - |
- |
- |
Nautilia profundicola | - |
- |
- |
Purification (Comment) | Organism |
---|---|
maltose binding protein Trap column chromatography | Campylobacter fetus |
maltose binding protein Trap column chromatography | Caminibacter mediatlanticus |
maltose binding protein Trap column chromatography | Nautilia profundicola |
maltose binding protein Trap column chromatography | Campylobacter curvus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1 | - |
nitrite reduction, pH 7.0, 37°C | Caminibacter mediatlanticus |
1.2 | - |
nitrite reduction, pH 7.0, 37°C | Nautilia profundicola |
27 | - |
nitrite reduction, pH 7.0, 37°C | Campylobacter curvus |
29 | - |
hydroxylamine reduction, pH 7.0, 37°C | Nautilia profundicola |
68 | - |
hydroxylamine reduction, pH 7.0, 37°C | Campylobacter curvus |
149 | - |
hydroxylamine reduction, pH 7.0, 37°C | Campylobacter fetus |
158 | - |
hydroxylamine reduction, pH 7.0, 37°C | Caminibacter mediatlanticus |
181 | - |
nitrite reduction, pH 7.0, 37°C | Campylobacter fetus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
Campylobacter fetus | nitrite + reduced 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
Caminibacter mediatlanticus | nitrite + reduced 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
Nautilia profundicola | nitrite + reduced 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
Campylobacter curvus | nitrite + reduced 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide + H2O | - |
Campylobacter fetus | NH3 + oxidized 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide + H2O | - |
Caminibacter mediatlanticus | NH3 + oxidized 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide + H2O | - |
Nautilia profundicola | NH3 + oxidized 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide + H2O | - |
Campylobacter curvus | NH3 + oxidized 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide | - |
? | |
hydroxylamine + H2O + 4 ferricytochrome c | - |
Campylobacter fetus | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
hydroxylamine + H2O + 4 ferricytochrome c | - |
Caminibacter mediatlanticus | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
hydroxylamine + H2O + 4 ferricytochrome c | - |
Nautilia profundicola | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
hydroxylamine + H2O + 4 ferricytochrome c | - |
Campylobacter curvus | nitrite + 4 ferrocytochrome c + 5 H+ | - |
? | |
hydroxylamine + phenazine methosulfate + H2O | - |
Campylobacter fetus | NH3 + oxidized phenazine methosulfate | - |
? | |
hydroxylamine + phenazine methosulfate + H2O | - |
Caminibacter mediatlanticus | NH3 + oxidized phenazine methosulfate | - |
? | |
hydroxylamine + phenazine methosulfate + H2O | - |
Nautilia profundicola | NH3 + oxidized phenazine methosulfate | - |
? | |
hydroxylamine + phenazine methosulfate + H2O | - |
Campylobacter curvus | NH3 + oxidized phenazine methosulfate | - |
? | |
additional information | hydroxylamine oxidation is negligible | Campylobacter fetus | ? | - |
? | |
additional information | hydroxylamine oxidation is negligible | Caminibacter mediatlanticus | ? | - |
? | |
additional information | hydroxylamine oxidation is negligible | Nautilia profundicola | ? | - |
? | |
additional information | hydroxylamine oxidation is negligible | Campylobacter curvus | ? | - |
? | |
nitrite + reduced benzyl viologen + H+ | - |
Campylobacter fetus | NH4+ + reduced methyl viologen + H2O | - |
? | |
nitrite + reduced benzyl viologen + H+ | - |
Caminibacter mediatlanticus | NH4+ + reduced methyl viologen + H2O | - |
? | |
nitrite + reduced benzyl viologen + H+ | - |
Nautilia profundicola | NH4+ + reduced methyl viologen + H2O | - |
? | |
nitrite + reduced benzyl viologen + H+ | - |
Campylobacter curvus | NH4+ + reduced methyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 80000, SDS-PAGE | Campylobacter fetus |
? | x * about 80000, SDS-PAGE | Caminibacter mediatlanticus |
? | x * about 80000, SDS-PAGE | Nautilia profundicola |
? | x * about 80000, SDS-PAGE | Campylobacter curvus |
Synonyms | Comment | Organism |
---|---|---|
HAO | - |
Campylobacter fetus |
HAO | - |
Caminibacter mediatlanticus |
HAO | - |
Nautilia profundicola |
HAO | - |
Campylobacter curvus |
haoA | - |
Campylobacter fetus |
haoA | - |
Caminibacter mediatlanticus |
haoA | - |
Nautilia profundicola |
haoA | - |
Campylobacter curvus |
hydroxylamine oxidoreductase | - |
Campylobacter fetus |
hydroxylamine oxidoreductase | - |
Caminibacter mediatlanticus |
hydroxylamine oxidoreductase | - |
Nautilia profundicola |
hydroxylamine oxidoreductase | - |
Campylobacter curvus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Campylobacter fetus | |
cytochrome c | - |
Caminibacter mediatlanticus | |
cytochrome c | - |
Nautilia profundicola | |
cytochrome c | - |
Campylobacter curvus | |
heme | - |
Campylobacter fetus | |
heme | - |
Caminibacter mediatlanticus | |
heme | - |
Nautilia profundicola | |
heme | - |
Campylobacter curvus |