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Literature summary for 1.7.2.5 extracted from

  • Bricio, C.; Alvarez, L.; San Martin, M.; Schurig-Briccio, L.A.; Gennis, R.B.; Berenguer, J.
    A third subunit in ancestral cytochrome c-dependent nitric oxide reductases (2014), Appl. Environ. Microbiol., 80, 4871-4878 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.2.5

Cloned(Commentary)

Cloned (Comment) Organism
genes norC and norB encoding the two enzyme subunits of enzyme cNor, and gene norH, a third gene enclosed into the gene cluster that is cotranscribed in a single mRNA from an inducible promoter. NorH constitutes a third subunit of the cNor, recombinant expression of His-tagged isolated NorH, of His-tagged wild-type enzyme cNOR, and of His-tagged NorH-deficient cNOR in Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics of recombinant wild-type and mutant enzymes Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane cNOR enzyme is integral to the membrane Thermus thermophilus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 nitric oxide + 2 ferrocytochrome c + 2 H+ Thermus thermophilus
-
 nitrous oxide + 2 ferricytochrome c + H2O
-
 ?
2 nitric oxide + 2 ferrocytochrome c + 2 H+ Thermus thermophilus PRQ25
-
 nitrous oxide + 2 ferricytochrome c + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus D5GU62 AND D5GU63 genes norC and norB encoding the two enzyme subunits
-
Thermus thermophilus PRQ25 D5GU62 AND D5GU63 genes norC and norB encoding the two enzyme subunits
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged NorH, wild-type cNOR, and mutant cNOR lacking norH by nickel affinity chromatography from Escherichia coli Thermus thermophilus

Source Tissue

Source Tissue Comment Organism Textmining
additional information Distribution of nor genes in diverse Thermus thermophilus strains, overview Thermus thermophilus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 nitric oxide + 2 ferrocytochrome c + 2 H+
-
 Thermus thermophilus nitrous oxide + 2 ferricytochrome c + H2O
-
 ?
2 nitric oxide + 2 ferrocytochrome c + 2 H+
-
 Thermus thermophilus PRQ25 nitrous oxide + 2 ferricytochrome c + H2O
-
 ?

Synonyms

Synonyms Comment Organism
cNOR
-
 Thermus thermophilus
cytochrome c type NOR
-
 Thermus thermophilus
cytochrome c-dependent nitric oxide reductase
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42 60 assay at Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
cytochrome c heme containing, dependent on, cytochrome c type NO reductase Thermus thermophilus

General Information

General Information Comment Organism
evolution the Nor enzymes belong to the same heme-copper oxidase superfamily as the O2-reducing cytochrome oxidases Thermus thermophilus
metabolism reduction of NO to N2O by denitrifiying bacteria is catalyzed either by a monomeric quinol-nitric oxide reductase (qNor, EC 1.7.5.2) or by a heterodimeric cytochrome c-dependent nitric oxide reductase (cNor), composed of three subunits encoded on single genes in one cotranscribed gene cluster, direct interactions between NorH and the NorCB complex. NOR are the main enzymes involved in the reduction of NO during the denitrification process Thermus thermophilus