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Literature summary for 1.7.2.5 extracted from
- Nitric oxide reductase from Pseudomonas stutzeri, a novel cytochrome bc complex. Phospholipid requirement, electron paramagnetic resonance and redox properties (1994), Eur. J. Biochem., 222, 293-303.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | - |
Pseudomonas stutzeri |  |
| phosphatidylglycerol | - |
Pseudomonas stutzeri | |
General Stability
| General Stability | Organism |
|---|---|
| a phospholipid is required for high catalytic activity | Pseudomonas stutzeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| elaidic acid | - |
Pseudomonas stutzeri | |
| linoleic acid | - |
Pseudomonas stutzeri | |
| linolenic acid | - |
Pseudomonas stutzeri | |
| oleic acid | - |
Pseudomonas stutzeri | |
| palmitoleic acid | - |
Pseudomonas stutzeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Pseudomonas stutzeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
1 * 17000 + 1 * 38000, SDS-PAGE | Pseudomonas stutzeri |
| 38000 | - |
1 * 17000 + 1 * 38000, SDS-PAGE | Pseudomonas stutzeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | Pseudomonas stutzeri | - |
nitrous oxide + acceptor + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
ZoBell strain, ATCC 14405 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| lipoprotein | contains cardiolipin, phosphatidylglycerol and phosphatidylethanolamine | Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 60 | - |
ascorbate and phenazine methosulfate as electron donors | Pseudomonas stutzeri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 17°C, 0.1% laurylpropanediol-3-phosphorylcholine ether or 0.1% dodecylmaltoside, 8 days, 100% activity | Pseudomonas stutzeri |
| 17°C, Triton X-100, 2 days, 100% activity | Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 nitric oxide + reduced acceptor | - |
Pseudomonas stutzeri | nitrous oxide + acceptor + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 17000 + 1 * 38000, SDS-PAGE | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | - |
- |
Pseudomonas stutzeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 6 | at pH 6.0 half of the activity of pH 4.8 | Pseudomonas stutzeri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
inactivation after 1 h at room temperature | Pseudomonas stutzeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Pseudomonas stutzeri | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
oleic acid | - |
Pseudomonas stutzeri | |
| 0.0098 | - |
palmitoleic acid | - |
Pseudomonas stutzeri | |
| 0.0125 | - |
linoleic acid | - |
Pseudomonas stutzeri | |
| 0.014 | - |
linolenic acid | - |
Pseudomonas stutzeri | |
| 0.0235 | - |
elaidic acid | - |
Pseudomonas stutzeri | |
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