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Literature summary for 1.7.2.4 extracted from

  • Sanchez, C.; Mitsui, H.; Minamisawa, K.
    Regulation of nitrous oxide reductase genes by NasT-mediated transcription antitermination in Bradyrhizobium diazoefficiens (2017), Environ. Microbiol. Rep., 9, 389-396 .
    View publication on PubMed
Search for more literature for 1.7.2.4

Cloned(Commentary)

Cloned (Comment) Organism
gene nosZ, expression as nosRZD transcript, quantitative PCR-based expression analysis Bradyrhizobium diazoefficiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrous oxide + 2 reduced cytochrome c Bradyrhizobium diazoefficiens
-
 nitrogen + H2O + 2 cytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium diazoefficiens Q89XJ6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrous oxide + 2 reduced cytochrome c
-
 Bradyrhizobium diazoefficiens nitrogen + H2O + 2 cytochrome c
-
 ?

Synonyms

Synonyms Comment Organism
nitrous oxide reductase
-
 Bradyrhizobium diazoefficiens
NosZ
-
 Bradyrhizobium diazoefficiens

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Bradyrhizobium diazoefficiens

Expression

Organism Comment Expression
Bradyrhizobium diazoefficiens the putative transcription antiterminator NasT is a positive regulator of nosZ; but in the absence of nitrate, NasT is counteracted by the nitrate sensor NasS down
Bradyrhizobium diazoefficiens in Bradyrhizobium diazoefficiens, maximal expression of the nitrous oxide reductase gene nosZ requires oxygen limitation and the presence of a nitrogen oxide. In contrast to nosRZD coding region, nosR-leader transcript level was not affected by nasS or nasT mutations under aerobic or denitrifying conditions respectively. Mapping of two transcription start sites of nosR and identification of two potential hairpins, H1 and H2, within the 5'-leader of nosR transcripts, the two-hairpin RNA structure acts for transcription termination upstream of nosR and the binding of NasT to H1 facilitates read-through transcription to induce nos expression additional information
Bradyrhizobium diazoefficiens the putative transcription antiterminator NasT is a positive regulator of nosZ, mechanism overview. But in the absence of nitrate, NasT is counteracted by the nitrate sensor NasS. NasT-mediated mechanism of nosRZDFYLX gene cluster expression, overview. NasT interacts specifically with nosR 5'-leader RNA and that the H1 hairpin contains the binding site critical for such interaction. The terminal loop of the recognition hairpin and the top base pair adjacent to it are important for the interaction with ANTAR proteins up

General Information

General Information Comment Organism
malfunction the level of nosRZD transcripts is severely impaired in the nasT mutant. In the nasT background, deletions of either hairpin leads to increased level of nosRZD transcripts. Under denitrifying conditions, level of nosR, nosZ and nosD transcripts in the nasT mutant severely decrease compared with the nasS mutant and the wild-type, as for nosZ. Phenotypic effects of deletions in the nosR leader region, overview Bradyrhizobium diazoefficiens
additional information in Bradyrhizobium diazoefficiens, maximal expression of the nitrous oxide reductase gene nosZ requires oxygen limitation and the presence of a nitrogen oxide. In contrast to nosRZD coding region, nosR-leader transcript level was not affected by nasS or nasT mutations under aerobic or denitrifying conditions respectively. Mapping of two transcription start sites of nosR and identification of two potential hairpins, H1 and H2, within the 5'-leader of nosR transcripts, the two-hairpin RNA structure acts for transcription termination upstream of nosR and the binding of NasT to H1 facilitates read-through transcription to induce nos expression Bradyrhizobium diazoefficiens