Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.2.4 extracted from

  • Sato, K.; Okubo, A.; Yamazaki, S.
    Characterization of a multi-copper enzyme, nitrous oxide reductase, from Rhodobacter sphaeroides f. sp. denitrificans (1998), J. Biochem., 124, 51-54.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 0.1 mM Cereibacter sphaeroides
Ni2+ 50% inhibition at 0.1 mM Cereibacter sphaeroides
Zn2+ complete inhibition at 0.1 mM Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Cu 7.0 atoms per dimer, needs 0.001 mM Cu2+ for full activity Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66180
-
2 * 66180, MALDI-TOF Cereibacter sphaeroides
67000
-
2 * 67000, SDS-PAGE Cereibacter sphaeroides
105000
-
gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nitrous oxide + reduced acceptor Cereibacter sphaeroides
-
nitrogen + H2O + acceptor
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
f. sp. denitrificans IL106
-

Purification (Commentary)

Purification (Comment) Organism
purification aerobically at 4°C Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
44
-
aerobic form Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrous oxide + reduced acceptor
-
Cereibacter sphaeroides nitrogen + H2O + acceptor
-
?
nitrous oxide + reduced acceptor photochemically-reduced benzyl viologen as electron donor Cereibacter sphaeroides nitrogen + H2O + acceptor
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 67000, SDS-PAGE Cereibacter sphaeroides
homodimer 2 * 66180, MALDI-TOF Cereibacter sphaeroides