Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | not inhibitory at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
Cu2+ | 50% inhibition at 0.1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
EDTA | not inhibitory at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
FAD | inhibition, when added to the standard assay mixture containing benzyl viologen as electron acceptor | Salmonella enterica subsp. enterica serovar Typhimurium | |
FMN | inhibition, when added to the standard assay mixture containing benzyl viologen as electron acceptor | Salmonella enterica subsp. enterica serovar Typhimurium | |
Hg2+ | 80% inhibition at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
KCN | not inhibitory at 5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
Mn2+ | not inhibitory at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
NaN3 | not inhibitory at 10 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.89 | - |
Trimethylamine N-oxide | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.2 | - |
Chlorate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
4 * 84000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
332000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
inducible enzyme | - |
Purification (Comment) | Organism |
---|---|
using polyethyleneimine to remove nucleic acids, ammonium sulfate treatment, column chromatography on DEAE-Sephacel and Bio-Gel A-5m, chromatofocusing and chromatography on Sephacryl-S-300 column | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
-80°C, 10% loss of activity after 15 days | Salmonella enterica subsp. enterica serovar Typhimurium |
0°C, 50 mM sodium phosphate buffer, pH 7.3, 30% loss of activity after 17 days | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorate + electron donor | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
additional information | when FMN, FAD, cytochrome c, NADPH, NADH or ascorbate are substituted at equimolar concentration for benzyl viologen, none facilitates the oxidation of dithionite by the trimethylamine N-oxide | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
additional information | the enzyme has very slight activity with nitrate, but no activity with nitrite, sulfite or thiosulfate | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Salmonella enterica subsp. enterica serovar Typhimurium | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + electron donor | the activity with 3.4 mM FMN is 17% of that obtained with 0.33 mM benzyl viologen | Salmonella enterica subsp. enterica serovar Typhimurium | trimethylamine + oxidized electron donor + H2O | - |
? | |
trimethylamine N-oxide + electron donor | NADH as electron donor | Salmonella enterica subsp. enterica serovar Typhimurium | trimethylamine + oxidized electron donor + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 84000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
10 min, pH 7.3, quite stable at temperatures below | Salmonella enterica subsp. enterica serovar Typhimurium |
80 | - |
10 min, pH 7.3, rapid loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
90 | - |
10 min, pH 7.3, complete loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.65 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.3 | - |
not stable | Salmonella enterica subsp. enterica serovar Typhimurium |
6 | 8.5 | stability peak at pH 7.3 | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Salmonella enterica subsp. enterica serovar Typhimurium |