Literature summary for 1.7.2.2 extracted from

Campecino, J.; Lagishetty, S.; Wawrzak, Z.; Sosa Alfaro, V.; Lehnert, N.; Reguera, G.; Hu, J.; Hegg, E.L.
Cytochrome c nitrite reductase from the bacterium Geobacter lovleyi represents a new NrfA subclass (2020), J. Biol. Chem., 295, 11455-11465 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Shewanella oneidensis	Trichlorobacter lovleyi

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure at 2.55 A resolution, presence of an arginine residue in the region otherwise occupied by calcium in canonical NrfA enzymes	Trichlorobacter lovleyi

Protein Variants

Protein Variants	Comment	Organism
R277A	variant contains all five hemes, less than 3% of wild-type activity	Trichlorobacter lovleyi
R277K	variant contains all five hemes, less than 3% of wild-type activity	Trichlorobacter lovleyi
R277Q	variant contains all five hemes, less than 3% of wild-type activity	Trichlorobacter lovleyi

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	not inhibitory: EDTA at 1 mM	Trichlorobacter lovleyi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.027	-	nitrite	pH 7, temperature not specified in the publication	Trichlorobacter lovleyi

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	not required: Ca2+	Trichlorobacter lovleyi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	dynamic light scattering	Trichlorobacter lovleyi

Organism

Organism	UniProt	Comment	Textmining
Trichlorobacter lovleyi	B3E641	-	-
Trichlorobacter lovleyi DSM 17278	B3E641	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, by Strep-tag II affinity chromatography followed by size-exclusion chromatography	Trichlorobacter lovleyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrite + reduced methyl viologen	-	Trichlorobacter lovleyi	NH3 + oxidized methyl viologen + H2O	-	?
nitrite + reduced methyl viologen	-	Trichlorobacter lovleyi DSM 17278	NH3 + oxidized methyl viologen + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 56196, electrospray ionization, consistent with the mass of the polypeptide chain plus the five heme cofactors but no calcium ion	Trichlorobacter lovleyi
More	the enzyme crystallizes as a dimer but remains monomeric in solution	Trichlorobacter lovleyi

Synonyms

Synonyms	Comment	Organism
Glov_1042	-	Trichlorobacter lovleyi
NrfA	-	Trichlorobacter lovleyi

Cofactor

Cofactor	Comment	Organism	Structure
heme	five hemes per monomer	Trichlorobacter lovleyi

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Release 2023.1 (January 2023)