Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
expression in Escherichia coli | Wolinella succinogenes |
Crystallization (Comment) | Organism |
---|---|
mutant H264N, to 2.15 A resolution. Homodimeric protein, the constellation of His/His and Lys-coordinated c-hemes is indistinguishable from those of the native enzyme | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
H264N | mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme | Escherichia coli |
H277N | mutant is unable to catalyze nitrite reduction but able to reduce hydroxylamine. The mutant simultaneously binds nitrite and electrons at the catalytic heme | Wolinella succinogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
nitrite | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
0.08 | - |
nitrite | wild-type, pH 7.0, temperature not specified in the publication | Wolinella succinogenes | |
2.01 | - |
hydroxylamine | wild-type, pH 7.0, temperature not specified in the publication | Wolinella succinogenes | |
2.03 | - |
hydroxylamine | mutant H264N, pH 7.0, temperature not specified in the publication | Wolinella succinogenes | |
30 | - |
hydroxylamine | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
170 | - |
hydroxylamine | mutant H264N, pH 7.0, temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABK9 | - |
- |
Wolinella succinogenes | Q9S1E5 | - |
- |
Wolinella succinogenes DSM 1740 | Q9S1E5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + reduced benzyl viologen | - |
Escherichia coli | ? + oxidized benzyl viologen | - |
? | |
hydroxylamine + reduced benzyl viologen | - |
Wolinella succinogenes | ? + oxidized benzyl viologen | - |
? | |
hydroxylamine + reduced benzyl viologen | - |
Wolinella succinogenes DSM 1740 | ? + oxidized benzyl viologen | - |
? | |
nitrite + reduced methyl viologen | - |
Escherichia coli | NH3 + H2O + oxidized methyl viologen | - |
? | |
nitrite + reduced methyl viologen | - |
Wolinella succinogenes | NH3 + H2O + oxidized methyl viologen | - |
? | |
nitrite + reduced methyl viologen | - |
Wolinella succinogenes DSM 1740 | NH3 + H2O + oxidized methyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome c-552 | - |
Escherichia coli |
cytochrome c-552 | - |
Wolinella succinogenes |
NrfA | - |
Escherichia coli |
NrfA | - |
Wolinella succinogenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
197 | - |
hydroxylamine | mutant H264N, pH 7.0, temperature not specified in the publication | Wolinella succinogenes | |
215 | - |
hydroxylamine | wild-type, pH 7.0, temperature not specified in the publication | Wolinella succinogenes | |
758 | - |
nitrite | wild-type, pH 7.0, temperature not specified in the publication | Wolinella succinogenes | |
1350 | - |
nitrite | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli | |
3100 | - |
hydroxylamine | mutant H264N, pH 7.0, temperature not specified in the publication | Escherichia coli | |
3160 | - |
hydroxylamine | wild-type, pH 7.0, temperature not specified in the publication | Escherichia coli |