Literature summary for 1.7.2.2 extracted from

Silveira, C.M.; Besson, S.; Moura, I.; Moura, J.J.; Almeida, M.G.
Measuring the cytochrome C nitrite reductase activity-practical considerations on the enzyme assays (2010), Bioinorg. Chem. Appl., 2010, 634597.

Search for more literature for 1.7.2.2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0027	-	reduced phenosafranine	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans
0.0064	-	reduced diquat	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans
0.015	-	reduced methyl viologen	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans

Organism

Organism	UniProt	Comment	Textmining
Desulfovibrio desulfuricans	Q8VNU2	catalytic subunit	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrite + reduced anthraquinone-2-sulfonate + H+	about 95% conversion	Desulfovibrio desulfuricans	NH3 + H2O + oxidized anthraquinone-2-sulfonate	-	?
nitrite + reduced diquat + H+	100% conversion	Desulfovibrio desulfuricans	NH3 + H2O + oxidized diquat	-	?
nitrite + reduced indigo carmine + H+	75% conversion	Desulfovibrio desulfuricans	NH3 + H2O + oxidized indigo carmine	-	?
nitrite + reduced methyl viologen + H+	about 98% conversion	Desulfovibrio desulfuricans	NH3 + H2O + oxidized methyl viologen	-	?
nitrite + reduced phenosafranine + H+	about 102% conversion	Desulfovibrio desulfuricans	NH3 + H2O + oxidized phenosafranine	-	?

Synonyms

Synonyms	Comment	Organism
ccNiR	-	Desulfovibrio desulfuricans
cytochrome c nitrite reductase	-	Desulfovibrio desulfuricans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
79	-	reduced phenosafranine	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans
738	-	reduced methyl viologen	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans
1064	-	reduced diquat	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Desulfovibrio desulfuricans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.029	-	reduced phenosafranine	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans
0.049	-	reduced methyl viologen	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans
0.1664	-	reduced diquat	apparent value, in 0.2 M phosphate buffer pH 7.6, at 37°C	Desulfovibrio desulfuricans

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Release 2023.1 (January 2023)