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Literature summary for 1.7.2.2 extracted from

  • Almeida, M.G.; Silveira, C.M.; Guigliarelli, B.; Bertrand, P.; Moura, J.J.; Moura, I.; Leger, C.
    A needle in a haystack: The active site of the membrane-bound complex cytochrome c nitrite reductase (2007), FEBS Lett., 581, 284-288.
    View publication on PubMed
Search for more literature for 1.7.2.2

Inhibitors

Inhibitors Comment Organism Structure
CO CO and nitrite both bind to the catalytic heme, bimolecular inhibition kinetics, overview; competitive inhibitor Desulfovibrio desulfuricans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, overview Desulfovibrio desulfuricans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Desulfovibrio desulfuricans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the NrfA2NrfH complex houses 14 hemes Desulfovibrio desulfuricans

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
 strain ATCC 27774
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information active site structure determination and analysis, proton transfer is coupled to electron transfer at the active site, overview Desulfovibrio desulfuricans ?
-
 ?
nitrite + reduced methyl viologen
-
 Desulfovibrio desulfuricans NH3 + oxidized methyl viologen + H2O
-
 ?
nitrite + reduced methyl viologen nitrite and inhibitor CO both bind to the catalytic heme Desulfovibrio desulfuricans NH3 + H2O + oxidized methyl viologen
-
 ?

Synonyms

Synonyms Comment Organism
ccNiR
-
 Desulfovibrio desulfuricans
cytochrome c nitrite reductase
-
 Desulfovibrio desulfuricans
NrfA2NrfH complex
-
 Desulfovibrio desulfuricans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Desulfovibrio desulfuricans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
340
-
 nitrite at pH 7.6, 20°C, in phosphate buffer Desulfovibrio desulfuricans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Desulfovibrio desulfuricans

Cofactor

Cofactor Comment Organism Structure
heme contains 14 hemes Desulfovibrio desulfuricans
heme the NrfA2NrfH complex houses 14 hemes, closely-spaced hemes in this enzyme with magnetic interactions between them Desulfovibrio desulfuricans
methyl viologen artificial cofactor Desulfovibrio desulfuricans