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Literature summary for 1.7.2.2 extracted from

  • Kajie, S.; Anraku, Y.
    Purification of a hexaheme cytochrome c552 from Escherichia coli K 12 and its properties as a nitrite reductase (1986), Eur. J. Biochem., 154, 457-463.
    View publication on PubMed
Search for more literature for 1.7.2.2

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 0.01 mM,70% inhibition; 0.1 mM, 69% inhibition Escherichia coli K-12
CuSO4 0.01 mM, 68% inhibition Escherichia coli K-12
KCN 0.1 mM, 84% inhibition; 1 mM, 100% Escherichia coli K-12
KNO3 1 mM, 6% inhibition Escherichia coli K-12
NaN3 1 mM, 16% inhibition Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 nitrite
-
 Escherichia coli K-12
18
-
 hydroxylamine
-
 Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no non-heme iron Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 SDS-PAGE Escherichia coli K-12
72000
-
 native enzyme, gel filtration Escherichia coli K-12

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
 ST249
-
Escherichia coli K-12 ST249
-
 ST249
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli K-12

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36
-
 FADH2 Escherichia coli K-12
50
-
 FMNH2 Escherichia coli K-12
2690
-
 hydroxylamine Escherichia coli K-12
2930
-
 nitrite Escherichia coli K-12
2930
-
 benzyl viologen Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxylamine + benzyl viologen reduced
-
 Escherichia coli K-12 ? + benzyl viologen oxidized
-
 ?
hydroxylamine + benzyl viologen reduced
-
 Escherichia coli K-12 ST249 ? + benzyl viologen oxidized
-
 ?
additional information no nitrate or sulfite reductase activity Escherichia coli K-12 ?
-
 ?
additional information not accepted electron donors: NADH, NADPH, ubiquinol-1, menadiol, formate, D-lactate, sn-glycerol-3-phosphate Escherichia coli K-12 ?
-
 ?
additional information no nitrate or sulfite reductase activity Escherichia coli K-12 ST249 ?
-
 ?
additional information not accepted electron donors: NADH, NADPH, ubiquinol-1, menadiol, formate, D-lactate, sn-glycerol-3-phosphate Escherichia coli K-12 ST249 ?
-
 ?
nitrite + benzyl viologen reduced + H+
-
 Escherichia coli K-12 NH4+ + benzyl viologen oxidized + H2O
-
 ?
nitrite + benzyl viologen reduced + H+
-
 Escherichia coli K-12 ST249 NH4+ + benzyl viologen oxidized + H2O
-
 ?
nitrite + FADH2
-
 Escherichia coli K-12 NH4+ + FAD
-
 ?
nitrite + FADH2
-
 Escherichia coli K-12 ST249 NH4+ + FAD
-
 ?
nitrite + FMNH2
-
 Escherichia coli K-12 NH4+ + FMN
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 69000, SDS-PAGE Escherichia coli K-12

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 activity increases up to 60 Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli K-12

Cofactor

Cofactor Comment Organism Structure
heme c
-
 Escherichia coli K-12