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Literature summary for 1.7.2.1 extracted from

  • Agrawal, S.; Gample, S.; Yeware, A.; Sarkar, D.
    Novel gene similar to nitrite reductase (NO forming) plays potentially important role in the latency of tuberculosis (2021), Sci. Rep., 11, 19813 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
azide 5 mM, 95.2% inhibition Mycobacterium tuberculosis
EDTA 5 mM, 99% inhibition Mycobacterium tuberculosis
additional information not inhibitory: tungstate and iNOS inhibitor 1400 W Mycobacterium tuberculosis
NADH 5 mM, 92.3% inhibition Mycobacterium tuberculosis
nitrate 5 mM, 47.4% inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.16
-
nitrite pH 7, 37°C Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis A5U4H6 cf. EC 3.5.4.4
-
Mycobacterium tuberculosis ATCC 25177 A5U4H6 cf. EC 3.5.4.4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitrite + reduced electron donor
-
Mycobacterium tuberculosis NO + H2O + oxidized electron donor
-
?
nitrite + reduced electron donor
-
Mycobacterium tuberculosis ATCC 25177 NO + H2O + oxidized electron donor
-
?

Subunits

Subunits Comment Organism
? x * 26000, calculated from sequence and SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
MRA2164
-
Mycobacterium tuberculosis
NirK
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100
-
nitrite pH 7, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Mycobacterium tuberculosis

Expression

Organism Comment Expression
Mycobacterium tuberculosis expression is significantly upregulated after nitrite exposure up

General Information

General Information Comment Organism
physiological function purified recombinant MRA2164 protein shows significant nitrite dependent NO synthesizing activity. The knockdown of the MRA2164 gene expression results in a significantly reduced NO level compared to the wild type bacilli with a simultaneous return of its replicative capability Mycobacterium tuberculosis