Literature summary for 1.7.2.1 extracted from

Fukuda, Y.; Matsusaki, T.; Tse, K.M.; Mizohata, E.; Murphy, M.E.P.; Inoue, T.
Crystallographic study of dioxygen chemistry in a copper-containing nitrite reductase from Geobacillus thermodenitrificans (2018), Acta crystallogr. Sect.D, 74, 769-777 .

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Geobacillus thermodenitrificans

Crystallization (Commentary)

Crystallization (Comment)	Organism
the dioxygen present in an aerobically manipulated crystal can bind to the catalytic type 2 copper site of NirK during anaerobic synchrotron-radiation crystallography experiments. The structure shows a dual conformation of one water molecule as an axial ligand in the type 2 copper site. In the structure of the C135A mutant with peroxide bound to the type 2 copper atom, the peroxide molecule is mainly observed in a side-on binding manner, with a possible minor end-on conformation	Geobacillus thermodenitrificans

Crystallization (Comment)

Organism

the dioxygen present in an aerobically manipulated crystal can bind to the catalytic type 2 copper site of NirK during anaerobic synchrotron-radiation crystallography experiments. The structure shows a dual conformation of one water molecule as an axial ligand in the type 2 copper site. In the structure of the C135A mutant with peroxide bound to the type 2 copper atom, the peroxide molecule is mainly observed in a side-on binding manner, with a possible minor end-on conformation

Geobacillus thermodenitrificans

Protein Variants

Protein Variants	Comment	Organism
C135A	in the anaerobic synchrotron-radiation crystallography structure with peroxide bound to the type 2 copper atom, the peroxide molecule is mainly observed in a side-on binding manner, with a possible minor end-on conformation	Geobacillus thermodenitrificans

Organism

Organism	UniProt	Comment	Textmining
Geobacillus thermodenitrificans	A0A1W6VP04	-	-

Synonyms

Synonyms	Comment	Organism
NirK	-	Geobacillus thermodenitrificans

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Release 2023.1 (January 2023)