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Literature summary for 1.7.2.1 extracted from

  • Sparacino-Watkins, C.E.; Tejero, J.; Sun, B.; Gauthier, M.C.; Thomas, J.; Ragireddy, V.; Merchant, B.A.; Wang, J.; Azarov, I.; Basu, P.; Gladwin, M.T.
    Nitrite reductase and nitric-oxide synthase activity of the mitochondrial molybdopterin enzymes mARC1 and mARC2 (2014), J. Biol. Chem., 289, 10345-10358 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and in HEK cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
C273A mutation in the putative active site cysteine residue, known to coordinate molybdenum binding. NO formation is abolished by the C273A mutation Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Tungsten replacement of molybdenum by tungsten abolishes NO formation Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.5
-
nitrite pH 7.4, temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Molybdenum nitrite reduction is catalyzed by molybdenum in the active site Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5VT66
-
-
Homo sapiens Q969Z3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mARC can generate nitric oxide from nitrite when forming an electron transfer chain with NADH, cytochrome b5, and NADH-dependent cytochrome b5 reductase Homo sapiens ?
-
?
nitrite + ferrocytochrome b5 + 2 H+
-
Homo sapiens nitric oxide + H2O + ferricytochrome b5
-
?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE, recombinant His-tagged protein lacking the mitochondrial translocation motif Homo sapiens

Synonyms

Synonyms Comment Organism
mARC1
-
Homo sapiens
mARC2
-
Homo sapiens
mitochondrial amidoxime reducing component
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
nitrite pH 7.4, temperature not specified in the publication Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
3fold decrease in the rate of NO formation compared to pH 6.5 Homo sapiens