Literature summary for 1.7.2.1 extracted from

Fukuda, Y.; Koteishi, H.; Yoneda, R.; Tamada, T.; Takami, H.; Inoue, T.; Nojiri, M.
Structural and functional characterization of the Geobacillus copper nitrite reductase involvement of the unique N-terminal region in the interprotein electron transfer with its redox partner (2014), Biochim. Biophys. Acta, 1837, 396-405 .

Cloned(Commentary)

Cloned (Comment)	Organism
-	Geobacillus kaustophilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of copper-containing nitrite reductase NiR and the N-terminal 68 residue-deleted mutant, at resolutions of 1.3 A and 1.8 A, respectively. Both structures show a striking resemblance with the overall structure of the well-known copper-containing nitrite reductases composed of two Greek key beta-barrel domains. The N-terminal region has one beta-strand and one alpha-helix extended to the northern surface of the type-1 copper site. This region contributes to the transient binding with the partner protein during the interprotein electron transfer reaction in the Geobacillus system. The region is directly involved in the specific partner recognition	Geobacillus kaustophilus

Crystallization (Comment)

Organism

structures of copper-containing nitrite reductase NiR and the N-terminal 68 residue-deleted mutant, at resolutions of 1.3 A and 1.8 A, respectively. Both structures show a striking resemblance with the overall structure of the well-known copper-containing nitrite reductases composed of two Greek key beta-barrel domains. The N-terminal region has one beta-strand and one alpha-helix extended to the northern surface of the type-1 copper site. This region contributes to the transient binding with the partner protein during the interprotein electron transfer reaction in the Geobacillus system. The region is directly involved in the specific partner recognition

Geobacillus kaustophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.079	-	ferricytochrome c551	pH 8.0, 25°C	Geobacillus kaustophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
copper	two His nitrogen atoms (His126 and His174), and two sulfur atoms, one from Cys166 and the other from Met179, coordinate the Cu atom	Geobacillus kaustophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus kaustophilus	Q5L1X8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitric oxide + H2O + ferricytochrome c551	-	Geobacillus kaustophilus	nitrite + ferrocytochrome c551 + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
GK0767	-	Geobacillus kaustophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
796	-	ferricytochrome c551	pH 8.0, 25°C	Geobacillus kaustophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
10100	-	ferricytochrome c551	pH 8.0, 25°C	Geobacillus kaustophilus