Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | 20% residual activity | Fusarium oxysporum | |
diethyldithiocarbamate | 1 mM, 10% residual activity | Fusarium oxysporum | |
EDTA | 1 mM, 60% resiudal activity | Fusarium oxysporum | |
KCN | 1 mM, 5% residual activity | Fusarium oxysporum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the first 65 amino acid residues of theN-terminal domain constitute a mitochondrial targeting signal, sequence analysis | Fusarium oxysporum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | ligands of the type 1 and 2 Cu centers are completely conserved in NiR. They are His253, Cys294, His302, and Met307 for the type 1 Cu center and His258, His293, and His456 for type 2.Asp256 and His405 residues are important for enzyme catalysis by forming a hydrogen bond network on the type 2 Cu center. The EPR spectrum is also characteristic of NirK proteins that contain both type 1 and type 2 copper centers | Fusarium oxysporum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53705 | - |
x * 53705, calculated, x * 60000, SDS-PAGE | Fusarium oxysporum |
60000 | - |
x * 53705, calculated, x * 60000, SDS-PAGE | Fusarium oxysporum |
900000 | - |
gel filtration, aggregate | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | A9XR52 | - |
- |
Purification (Comment) | Organism |
---|---|
purified NiR exhibits a blue color spectrum showing maximum absorbance at 594 nm | Fusarium oxysporum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
303 | - |
pH 6.5, 35°C | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + methyl viologen | - |
Fusarium oxysporum | NO + oxidized methyl viologen + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53705, calculated, x * 60000, SDS-PAGE | Fusarium oxysporum |
Synonyms | Comment | Organism |
---|---|---|
NirK | - |
Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
optimum below pH 6.5 | Fusarium oxysporum |