Crystallization (Comment) | Organism |
---|---|
crystals of Y25S mutant protein are grown from a solution containing 10-20 mg/ml protein in the presence of 2.2-2.4 M ammonium sulfate and 50 mM potassium phosphate, pH 7.0, crystals diffract to 1.4 A | Paracoccus pantotrophus |
Protein Variants | Comment | Organism |
---|---|---|
Y25S | unlike the wild-type enzyme, the Y25S mutant is active as a reductase toward nitrite, O2, and hydroxylamine without a reduuctive activation step | Paracoccus pantotrophus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
nitrite | 25°C, pH 7.0, Y25S mutant enzyme | Paracoccus pantotrophus | |
0.071 | - |
nitrite | 25°C, pH 7.0, activated wild-type enzyme | Paracoccus pantotrophus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | heme containing enzyme | Paracoccus pantotrophus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63022 | - |
2 * 63022, electrospray mass spectroscopy | Paracoccus pantotrophus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus pantotrophus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose, Poros HP2, Superdex 200 | Paracoccus pantotrophus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxylamine + reduced pseudoazurin | - |
Paracoccus pantotrophus | NH3 + H2O + oxidized pseudoazurin | - |
? | |
nitrite + reduced pseudoazurin | - |
Paracoccus pantotrophus | NO + H2O + oxidized pseudoazurin | - |
? | |
O2 + reduced pseudoazurin | - |
Paracoccus pantotrophus | H2O + oxidized pseudoazurin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63022, electrospray mass spectroscopy | Paracoccus pantotrophus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
nitrite | 25°C, pH 7.0, wild-type, without pre-reduction with dithionite | Paracoccus pantotrophus | |
3.2 | - |
O2 | 25°C, pH 7.0, wild-type, pre-reduction with dithionite | Paracoccus pantotrophus | |
3.2 | - |
hydroxylamine | 25°C, pH 7.0, wild-type, pre-reduction with dithionite | Paracoccus pantotrophus | |
6.2 | - |
O2 | 25°C, pH 7.0, Y25S mutant enzyme | Paracoccus pantotrophus | |
7.7 | - |
hydroxylamine | 25°C, pH 7.0, Y25S mutant enzyme | Paracoccus pantotrophus | |
67 | - |
nitrite | 25°C, pH 7.0, Y25S mutant enzyme | Paracoccus pantotrophus | |
68 | - |
nitrite | 25°C, pH 7.0, wild-type, pre-reduction with dithionite | Paracoccus pantotrophus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme c | - |
Paracoccus pantotrophus | |
Heme d1 | - |
Paracoccus pantotrophus |