Cloned (Comment) | Organism |
---|---|
D92E and D92N mutant enzyme | Achromobacter xylosoxidans |
Protein Variants | Comment | Organism |
---|---|---|
D92E | mutation in type 2 Cu center, very low activity with artificial electron donors methyl viologen and sodium dithionite, 20-30% of wild-type activity with physiological electron donor azurin I | Achromobacter xylosoxidans |
D92N | mutation in type 2 Cu center, very low activity with artificial electron donors methyl viologen and sodium dithionite, 60-70% of wild-type activity with physiological electron donor azurin I | Achromobacter xylosoxidans |
H139A | mutation in type 1 Cu center, very low activity with the artificial electron donor methyl viologen, no activity with the physiological electron donor azurin I | Achromobacter xylosoxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | the type 1 Cu center functions as electron acceptor from the physiological electron donor azurin I, the type 2 Cu center is involved in binding and reduction of the substrate | Achromobacter xylosoxidans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced azurin + O2 | Achromobacter xylosoxidans | putative physiological electron donor | oxidized azurin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter xylosoxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and D92E and D92N mutant enzyme | Achromobacter xylosoxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45.2 | - |
native recombinant enzyme, azurin | Achromobacter xylosoxidans |
107 | - |
native recombinant enzyme, dithionite | Achromobacter xylosoxidans |
168 | - |
native recombinant enzyme, methyl viologen | Achromobacter xylosoxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrite + ferrocytochrome c | - |
Achromobacter xylosoxidans | nitric oxide + H2O + ferricytochrome c | - |
? | |
NO2- + reduced azurin | putative physiological electron donor | Achromobacter xylosoxidans | NO + oxidized azurin | - |
? | |
reduced azurin + O2 | putative physiological electron donor | Achromobacter xylosoxidans | oxidized azurin + H2O | - |
? |