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Literature summary for 1.7.2.1 extracted from

  • Cutruzzola, f.; Arese, M.; Grasso, S.; Bellelli, A.; Brunori, M.
    Mutagenesis of nitrite reductase from Pseudomonas aeruginosa: tyrosine-10 in the c heme domain is not involved in catalysis (1997), FEBS Lett., 412, 365-369.
    View publication on PubMed
Search for more literature for 1.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and Y10F mutant enzyme in Pseudomonas putida Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
Y10F no change in optical spectrum, nitrite and oxidase activity and heme c to heme d1 electron transfer rates compared to wild-type Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00084
-
 azurin recombinant Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
0.00177
-
 ferricytochrome c551 recombinant wild-type enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
0.035
-
 azurin recombinant wild-type and Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and Y10F mutant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nitric oxide + H2O + ferricytochrome c551
-
 Pseudomonas aeruginosa nitrite + ferrocytochrome c551 + H+
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.583
-
 ferricytochrome c551 recombinant wild-type enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
0.6
-
 ferricytochrome c551 recombinant Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
1
-
 azurin recombinant Y10F mutant enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa
1.12
-
 azurin recombinant wild-type enzyme, at pH 6.2 and 27°C Pseudomonas aeruginosa