Cloned (Comment) | Organism |
---|---|
gene nfsA, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics of recombinant His6-tagged enzyme | Escherichia coli | |
0.029 | - |
2,4,6-trinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli | |
0.3257 | - |
2-amino-4,6-dinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli | |
0.6427 | - |
4-amino-2,6-dinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,6-trinitrotoluene + NADPH + H+ | Escherichia coli | - |
4-hydroxylamino-2,6-dinitrotoluene + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P17117 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to over 95% purity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4,6-trinitrotoluene + NADPH + H+ | - |
Escherichia coli | 4-hydroxylamino-2,6-dinitrotoluene + NADP+ + H2O | - |
? | |
2,4,6-trinitrotoluene + NADPH + H+ | i.e. TNT, NfsA reduces TNT to the para isomer 4-hydroxylamino-2,6-dinitrotoluene more easily than the ortho isomer 2-hydroxylamino-4,6-dinitrotoluene | Escherichia coli | 4-hydroxylamino-2,6-dinitrotoluene + NADP+ + H2O | - |
? | |
2-amino-4,6-dinitrotoluene + NADPH + H+ | - |
Escherichia coli | ? + NADP+ + H2O | - |
? | |
4-amino-2,6-dinitrotoluene + NADPH + H+ | - |
Escherichia coli | ? + NADP+ + H2O | - |
? | |
additional information | no activity with 2,4-diamino-6-nitrotoluene and 2,6-diamino-4-nitrotoluene | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NfsA | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
4-amino-2,6-dinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli | |
12.36 | - |
2-amino-4,6-dinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli | |
56.2 | - |
2,4,6-trinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
evolution | nitroreductases are a family of flavoproteins that catalyze the NAD(P)H-dependent reduction of nitro groups to hydroxylamino and/or amino groups on various nitro-substituted compounds | Escherichia coli |
additional information | the enzyme crystal structure of NfsA, PDB ID 1F5V, is used for molecular ligand docking studies | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
4-amino-2,6-dinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli | |
38 | - |
2-amino-4,6-dinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli | |
1931 | - |
2,4,6-trinitrotoluene | pH 7.0, 37°C, recombinant His6-tagged enzyme | Escherichia coli |