Literature summary for 1.7.1.B3 extracted from

Green, L.K.; Syddall, S.P.; Carlin, K.M.; Bell, G.D.; Guise, C.P.; Mowday, A.M.; Hay, M.P.; Smaill, J.B.; Patterson, A.V.; Ackerley, D.F.
Pseudomonas aeruginosa NfsB and nitro-CBI-DEI - a promising enzyme/prodrug combination for gene directed enzyme prodrug therapy (2013), Mol. Cancer, 12, 58 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene nfsA, recombinant overexpression in Escherichia coli reporter strain SOS-R2, which contains a lacZ reporter gene under control of the SOS responsive promoter sfiA, functional nitroreductase NfsA expression in human HCT-116 cells	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P17117	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+	i.e. CB1954, an aziridinyl dinitrobenzamide prodrug	Escherichia coli	? + NADP+	-	?
additional information	an intrinsically oxygen-insensitive nature of the two-electron reduction mechanism	Escherichia coli	?	-	?
nitro-CBI-5-[(dimethylamino)ethoxy]indole + NAD(P)H + H+	a nitro-CBI prodrug, low activity	Escherichia coli	? + NAD(P)+	-	?

Synonyms

Synonyms	Comment	Organism
NfsA	-	Escherichia coli
nitroreductase A	-	Escherichia coli

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Release 2023.1 (January 2023)