BRENDA - Enzyme Database show
show all sequences of 1.7.1.B3

Pseudomonas aeruginosa NfsB and nitro-CBI-DEI - a promising enzyme/prodrug combination for gene directed enzyme prodrug therapy

Green, L.K.; Syddall, S.P.; Carlin, K.M.; Bell, G.D.; Guise, C.P.; Mowday, A.M.; Hay, M.P.; Smaill, J.B.; Patterson, A.V.; Ackerley, D.F.; Mol. Cancer 12, 58 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene nfsA, recombinant overexpression in Escherichia coli reporter strain SOS-R2, which contains a lacZ reporter gene under control of the SOS responsive promoter sfiA, functional nitroreductase NfsA expression in human HCT-116 cells
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P17117
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+
i.e. CB1954, an aziridinyl dinitrobenzamide prodrug
743280
Escherichia coli
? + NADP+
-
-
-
?
additional information
an intrinsically oxygen-insensitive nature of the two-electron reduction mechanism
743280
Escherichia coli
?
-
-
-
-
nitro-CBI-5-[(dimethylamino)ethoxy]indole + NAD(P)H + H+
a nitro-CBI prodrug, low activity
743280
Escherichia coli
? + NAD(P)+
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
gene nfsA, recombinant overexpression in Escherichia coli reporter strain SOS-R2, which contains a lacZ reporter gene under control of the SOS responsive promoter sfiA, functional nitroreductase NfsA expression in human HCT-116 cells
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+
i.e. CB1954, an aziridinyl dinitrobenzamide prodrug
743280
Escherichia coli
? + NADP+
-
-
-
?
additional information
an intrinsically oxygen-insensitive nature of the two-electron reduction mechanism
743280
Escherichia coli
?
-
-
-
-
nitro-CBI-5-[(dimethylamino)ethoxy]indole + NAD(P)H + H+
a nitro-CBI prodrug, low activity
743280
Escherichia coli
? + NAD(P)+
-
-
-
?
Other publictions for EC 1.7.1.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741786
Valiauga
Reduction of quinones and nit ...
Escherichia coli
Arch. Biochem. Biophys.
614
14-22
2017
-
2
-
-
-
-
2
13
-
-
-
-
-
1
-
-
-
1
-
-
-
-
13
-
1
-
-
12
1
-
-
2
2
-
-
-
2
-
2
-
-
-
-
2
2
13
-
-
-
-
-
-
-
-
-
-
-
-
13
-
1
-
-
12
1
-
-
-
-
2
2
-
12
12
743709
Bai
Transformation pathway of 2,4 ...
Escherichia coli
Process Biochem.
50
705-711
2015
-
-
1
-
-
-
-
4
-
-
-
1
-
1
-
-
1
-
-
-
-
-
5
-
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
5
-
1
-
-
3
1
-
-
-
-
2
2
-
3
3
742167
Yang
Residue Phe42 is critical for ...
Escherichia coli
Biotechnol. Lett.
35
1693-1700
2013
-
-
1
-
3
-
1
19
-
-
-
-
-
1
1
-
1
1
-
-
4
-
5
1
1
-
-
20
1
-
-
2
-
-
-
-
-
1
2
-
3
-
-
1
-
19
-
-
-
-
-
1
-
1
-
-
4
-
5
1
1
-
-
20
1
-
-
-
-
1
1
-
20
20
743280
Green
Pseudomonas aeruginosa NfsB a ...
Escherichia coli
Mol. Cancer
12
58
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742109
Zenno
Purification and characetriza ...
Bacillus subtilis, Bacillus subtilis ISW 1214
Biosci. Biotechnol. Biochem.
62
1978-1987
1998
-
-
1
-
-
-
-
3
-
-
1
1
-
2
-
-
1
1
-
-
1
-
3
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
3
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-