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show all sequences of 1.7.1.B3

Residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA

Yang, J.; Zhan, J.; Bai, J.; Liu, P.; Xue, Y.; Yang, Q.; Biotechnol. Lett. 35, 1693-1700 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene nfsA, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
F42A
site-directed mutagenesis, the mutant enzymes shows 99% reduced activity toward nitrofurazone compared to the wild-type, and loss of helix content
Escherichia coli
F42N
site-directed mutagenesis, the mutant enzymes shows 96% reduced activity toward nitrofurazone compared to the wild-type, and loss of helix content
Escherichia coli
F42Y
site-directed mutagenesis, the mutant enzymes shows 52% reduced activity toward nitrofurazone compared to the wild-type
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
the enzyme is O2-insensitive
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten steady-state kinetics
Escherichia coli
0.012
-
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.013
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.02
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.022
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.023
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.029
-
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.049
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.054
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.064
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.085
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C; recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.176
-
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.206
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.313
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.322
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.327
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.332
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.366
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.487
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P17117
-
-
Oxidation Stability
Oxidation Stability
Organism
the enzyme is O2-insensitive
Escherichia coli
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Escherichia coli
Reaction
Reaction
Commentary
Organism
an aromatic amine + 3 NADP+ + 2 H2O = an aromatic nitrate + 3 NADPH + 3 H+
the enzyme uses NADPH as the hydrogen donor to catalyze the reduction of a nitro group by a ping-pong Bi-Bi kinetic mechanism
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.1
-
purified recombinant mutant F46A, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
0.29
-
purified recombinant mutant F46N, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
3.74
-
purified recombinant mutant F46Y, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
7.75
-
purified recombinant wild-type enzyme, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,6-trinitrotoluene + NADPH + H+
-
742167
Escherichia coli
? + NADP+ + H2O
-
-
-
?
2,4-dinitrotoluene + NADPH + H+
-
742167
Escherichia coli
? + NADP+ + H2O
-
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+
i.e. CB1954, an aziridinyl dinitrobenzamide prodrug
742167
Escherichia coli
? + NADP+
-
-
-
?
additional information
NfsA has a broad substrate spectrum. Various nitroaromatic substrates, including 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954), 2,4,6-trinitrotoluene (TNT), and 2,4-dinitrotoluene (2,4-DNT), can serve as substrates
742167
Escherichia coli
?
-
-
-
-
nitrofurazone + NADPH + H+
-
742167
Escherichia coli
5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
-
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.46
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.67
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.7
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.78
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.8
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1.08
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1.82
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
2.99
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
3.64
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
4.95
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
9.01
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
15.73
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
16.3
-
NADPH
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
17.31
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
19.37
-
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
24.27
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
24.94
-
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
27.06
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
36.64
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
56.21
-
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
a flavoenzyme which contains FMN as the prosthetic group
Escherichia coli
NADPH
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene nfsA, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
a flavoenzyme which contains FMN as the prosthetic group
Escherichia coli
NADPH
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F42A
site-directed mutagenesis, the mutant enzymes shows 99% reduced activity toward nitrofurazone compared to the wild-type, and loss of helix content
Escherichia coli
F42N
site-directed mutagenesis, the mutant enzymes shows 96% reduced activity toward nitrofurazone compared to the wild-type, and loss of helix content
Escherichia coli
F42Y
site-directed mutagenesis, the mutant enzymes shows 52% reduced activity toward nitrofurazone compared to the wild-type
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
the enzyme is O2-insensitive
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten steady-state kinetics
Escherichia coli
0.012
-
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.013
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.02
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.022
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.023
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.029
-
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.049
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.054
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.064
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.085
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C; recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.176
-
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.206
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.313
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.322
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.327
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
0.332
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.366
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.487
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
Oxidation Stability (protein specific)
Oxidation Stability
Organism
the enzyme is O2-insensitive
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.1
-
purified recombinant mutant F46A, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
0.29
-
purified recombinant mutant F46N, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
3.74
-
purified recombinant mutant F46Y, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
7.75
-
purified recombinant wild-type enzyme, pH 7.0, 22°C, substrate nitrofurazone
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,4,6-trinitrotoluene + NADPH + H+
-
742167
Escherichia coli
? + NADP+ + H2O
-
-
-
?
2,4-dinitrotoluene + NADPH + H+
-
742167
Escherichia coli
? + NADP+ + H2O
-
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + NADPH + H+
i.e. CB1954, an aziridinyl dinitrobenzamide prodrug
742167
Escherichia coli
? + NADP+
-
-
-
?
additional information
NfsA has a broad substrate spectrum. Various nitroaromatic substrates, including 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954), 2,4,6-trinitrotoluene (TNT), and 2,4-dinitrotoluene (2,4-DNT), can serve as substrates
742167
Escherichia coli
?
-
-
-
-
nitrofurazone + NADPH + H+
-
742167
Escherichia coli
5-(hydroxyamino)furan-2-carbaldehyde semicarbazone + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
-
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
22
-
assay at room temperature
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.46
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.67
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.7
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.78
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
0.8
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1.08
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1.82
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
2.99
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
3.64
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
4.95
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
9.01
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
15.73
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
16.3
-
NADPH
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
17.31
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
19.37
-
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
24.27
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
24.94
-
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
27.06
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
36.64
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
56.21
-
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Escherichia coli
General Information
General Information
Commentary
Organism
physiological function
residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA, Phe42 is important for maintaining NfsA activity and structure
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA, Phe42 is important for maintaining NfsA activity and structure
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
2
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
5
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
9
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
10
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
12
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
16
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
50
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
75
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
80
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
84
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
112
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
142
-
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
167
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
191.7
-
NADPH
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
250
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
720
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1350
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1610
-
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
1940
-
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
2
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
5
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
9
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
10
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
12
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
16
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
50
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
75
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
80
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
84
-
2,4-dinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
112
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
142
-
2,4-dinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
167
-
NADPH
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
191.7
-
NADPH
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
250
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
720
-
nitrofurazone
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1350
-
2,4,6-trinitrotoluene
recombinant mutant F46Y, pH 7.0, 22°C
Escherichia coli
1610
-
nitrofurazone
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
1940
-
2,4,6-trinitrotoluene
recombinant wild-type enzyme, pH 7.0, 22°C
Escherichia coli
Other publictions for EC 1.7.1.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741786
Valiauga
Reduction of quinones and nit ...
Escherichia coli
Arch. Biochem. Biophys.
614
14-22
2017
-
2
-
-
-
-
2
13
-
-
-
-
-
1
-
-
-
1
-
-
-
-
13
-
1
-
-
12
1
-
-
2
2
-
-
-
2
-
2
-
-
-
-
2
2
13
-
-
-
-
-
-
-
-
-
-
-
-
13
-
1
-
-
12
1
-
-
-
-
2
2
-
12
12
743709
Bai
Transformation pathway of 2,4 ...
Escherichia coli
Process Biochem.
50
705-711
2015
-
-
1
-
-
-
-
4
-
-
-
1
-
1
-
-
1
-
-
-
-
-
5
-
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
-
-
5
-
1
-
-
3
1
-
-
-
-
2
2
-
3
3
742167
Yang
Residue Phe42 is critical for ...
Escherichia coli
Biotechnol. Lett.
35
1693-1700
2013
-
-
1
-
3
-
1
19
-
-
-
-
-
1
1
-
1
1
-
-
4
-
5
1
1
-
-
20
1
-
-
2
-
-
-
-
-
1
2
-
3
-
-
1
-
19
-
-
-
-
-
1
-
1
-
-
4
-
5
1
1
-
-
20
1
-
-
-
-
1
1
-
20
20
743280
Green
Pseudomonas aeruginosa NfsB a ...
Escherichia coli
Mol. Cancer
12
58
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742109
Zenno
Purification and characetriza ...
Bacillus subtilis, Bacillus subtilis ISW 1214
Biosci. Biotechnol. Biochem.
62
1978-1987
1998
-
-
1
-
-
-
-
3
-
-
1
1
-
2
-
-
1
1
-
-
1
-
3
1
1
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
-
3
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-