Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Vibrio vulnificus |
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrotoluene | - |
Bacillus subtilis | |
2,4-dinitrotoluene | - |
Vibrio vulnificus | |
2,6-dinitrotoluene | - |
Bacillus subtilis | |
2,6-dinitrotoluene | - |
Vibrio vulnificus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
2,4-dinitrotoluene | at pH 8.0 and 30°C | Bacillus subtilis | |
0.32 | - |
2,4-dinitrotoluene | at pH 8.0 and 30°C | Vibrio vulnificus | |
0.51 | - |
2,6-dinitrotoluene | at pH 8.0 and 30°C | Bacillus subtilis | |
1.6 | - |
2,6-dinitrotoluene | at pH 8.0 and 30°C | Vibrio vulnificus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADPH + H+ | Vibrio vulnificus | - |
? | - |
? | |
2,4-dinitrotoluene + NADPH + H+ | Bacillus subtilis | highest activity | ? | - |
? | |
2,4-dinitrotoluene + NADPH + H+ | Vibrio vulnificus ATCC 27562 | - |
? | - |
? | |
2,4-dinitrotoluene + NADPH + H+ | Bacillus subtilis 168 | highest activity | ? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | Bacillus subtilis | - |
? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | Vibrio vulnificus | highest activity | ? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | Vibrio vulnificus ATCC 27562 | highest activity | ? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | Bacillus subtilis 168 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O34475 | - |
- |
Bacillus subtilis 168 | O34475 | - |
- |
Vibrio vulnificus | Q7MCD2 | - |
- |
Vibrio vulnificus ATCC 27562 | Q7MCD2 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Vibrio vulnificus |
Ni-NTA resin column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADPH + H+ | - |
Vibrio vulnificus | ? | - |
? | |
2,4-dinitrotoluene + NADPH + H+ | highest activity | Bacillus subtilis | ? | - |
? | |
2,4-dinitrotoluene + NADPH + H+ | - |
Vibrio vulnificus ATCC 27562 | ? | - |
? | |
2,4-dinitrotoluene + NADPH + H+ | highest activity | Bacillus subtilis 168 | ? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | - |
Bacillus subtilis | ? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | highest activity | Vibrio vulnificus | ? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | highest activity | Vibrio vulnificus ATCC 27562 | ? | - |
? | |
2,6-dinitrotoluene + NADPH + H+ | - |
Bacillus subtilis 168 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NfsB | - |
Vibrio vulnificus |
YfkO | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
2,6-dinitrotoluene | at pH 8.0 and 30°C | Bacillus subtilis | |
90 | - |
2,6-dinitrotoluene | at pH 8.0 and 30°C | Vibrio vulnificus | |
140 | - |
2,4-dinitrotoluene | at pH 8.0 and 30°C | Bacillus subtilis | |
200 | - |
2,4-dinitrotoluene | at pH 8.0 and 30°C | Vibrio vulnificus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.33 | - |
at pH 8.0 and 30°C | Bacillus subtilis | 2,4-dinitrotoluene | |
0.51 | - |
at pH 8.0 and 30°C | Vibrio vulnificus | 2,4-dinitrotoluene | |
0.67 | - |
at pH 8.0 and 30°C | Bacillus subtilis | 2,6-dinitrotoluene | |
0.8 | - |
at pH 8.0 and 30°C | Vibrio vulnificus | 2,6-dinitrotoluene |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
2,6-dinitrotoluene | at pH 8.0 and 30°C | Bacillus subtilis | |
57 | - |
2,6-dinitrotoluene | at pH 8.0 and 30°C | Vibrio vulnificus | |
640 | - |
2,4-dinitrotoluene | at pH 8.0 and 30°C | Vibrio vulnificus |