Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.7.1.B2 extracted from

  • Williams, E.; Sharrock, A.; Rylott, E.; Bruce, N.; MacKichan, J.; Ackerley, D.
    A cofactor consumption screen identifies promising NfsB family nitroreductases for dinitrotoluene remediation (2019), Biotechnol. Lett., 41, 1155-1162 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Vibrio vulnificus
expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrotoluene
-
Bacillus subtilis
2,4-dinitrotoluene
-
Vibrio vulnificus
2,6-dinitrotoluene
-
Bacillus subtilis
2,6-dinitrotoluene
-
Vibrio vulnificus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.062
-
2,4-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
0.32
-
2,4-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus
0.51
-
2,6-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
1.6
-
2,6-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-dinitrotoluene + NADPH + H+ Vibrio vulnificus
-
?
-
?
2,4-dinitrotoluene + NADPH + H+ Bacillus subtilis highest activity ?
-
?
2,4-dinitrotoluene + NADPH + H+ Vibrio vulnificus ATCC 27562
-
?
-
?
2,4-dinitrotoluene + NADPH + H+ Bacillus subtilis 168 highest activity ?
-
?
2,6-dinitrotoluene + NADPH + H+ Bacillus subtilis
-
?
-
?
2,6-dinitrotoluene + NADPH + H+ Vibrio vulnificus highest activity ?
-
?
2,6-dinitrotoluene + NADPH + H+ Vibrio vulnificus ATCC 27562 highest activity ?
-
?
2,6-dinitrotoluene + NADPH + H+ Bacillus subtilis 168
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O34475
-
-
Bacillus subtilis 168 O34475
-
-
Vibrio vulnificus Q7MCD2
-
-
Vibrio vulnificus ATCC 27562 Q7MCD2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Vibrio vulnificus
Ni-NTA resin column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrotoluene + NADPH + H+
-
Vibrio vulnificus ?
-
?
2,4-dinitrotoluene + NADPH + H+ highest activity Bacillus subtilis ?
-
?
2,4-dinitrotoluene + NADPH + H+
-
Vibrio vulnificus ATCC 27562 ?
-
?
2,4-dinitrotoluene + NADPH + H+ highest activity Bacillus subtilis 168 ?
-
?
2,6-dinitrotoluene + NADPH + H+
-
Bacillus subtilis ?
-
?
2,6-dinitrotoluene + NADPH + H+ highest activity Vibrio vulnificus ?
-
?
2,6-dinitrotoluene + NADPH + H+ highest activity Vibrio vulnificus ATCC 27562 ?
-
?
2,6-dinitrotoluene + NADPH + H+
-
Bacillus subtilis 168 ?
-
?

Synonyms

Synonyms Comment Organism
NfsB
-
Vibrio vulnificus
YfkO
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22
-
2,6-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
90
-
2,6-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus
140
-
2,4-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
200
-
2,4-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.33
-
at pH 8.0 and 30°C Bacillus subtilis 2,4-dinitrotoluene
0.51
-
at pH 8.0 and 30°C Vibrio vulnificus 2,4-dinitrotoluene
0.67
-
at pH 8.0 and 30°C Bacillus subtilis 2,6-dinitrotoluene
0.8
-
at pH 8.0 and 30°C Vibrio vulnificus 2,6-dinitrotoluene

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
43
-
2,6-dinitrotoluene at pH 8.0 and 30°C Bacillus subtilis
57
-
2,6-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus
640
-
2,4-dinitrotoluene at pH 8.0 and 30°C Vibrio vulnificus