Cloned (Comment) | Organism |
---|---|
gene LMJF_17_0725, DNA and amino acid sequence determination and analysis, , the gene has a 131 amino acid insertion encoding two tandem CBS domains, sequence comparisons, recombinant expression in Escherichia coli strain H1174 | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
IMP | competitive versus GMP | Leishmania major | |
Mycophenolic acid | competitive versus NADPH | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis | Leishmania major |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + NADPH + H+ | Leishmania major | - |
IMP + NH3 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q4QEB3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant untagged enzyme from Escherichia coli strain H1174 by affinity chromatography | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + NADPH + H+ | - |
Leishmania major | IMP + NH3 + NADP+ | - |
? | |
GMP + NADPH + H+ | reductive deamination of GMP, the back conversion of IMP to GMP is highly unfavorable | Leishmania major | IMP + NH3 + NADP+ | - |
? | |
additional information | enzyme nucleotide bindng analysis, nucleotide binding alters the quarternary structure of the enzyme, overview | Leishmania major | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | quaternary structure determination, LmGMPR subunits may adopt conformations with Trp121 differentially exposed to the aqueous environment | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
GMPR | - |
Leishmania major |
guanosine 5'-monophosphate reductase | - |
Leishmania major |
LmGMPR | - |
Leishmania major |
Lmj17.0725 | - |
Leishmania major |
LMJF_17_0725 | - |
Leishmania major |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
assay at | Leishmania major |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADH | Leishmania major | |
NADPH | binding analysis | Leishmania major |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
IMP | pH 6.9, 25°C, recombinant enzyme | Leishmania major | |
0.02 | - |
Mycophenolic acid | pH 6.9, 25°C, recombinant enzyme | Leishmania major |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of the cystathionine-beta-synthase, CBS, domain may impair the catalytic activity of mutant lmgmprDELTACBS and/or cause a disruption of the protein structure | Leishmania major |
additional information | LmGMPR subunits may adopt conformations with Trp121 differentially exposed to the aqueous environment, binding of ATP resulted in an emission spectrum with a lambdamax centered at 350 nm indicative of a conformational change that position Trp121 into a more solvent exposed environment | Leishmania major |
physiological function | the cystathionine-beta-synthase domains on the guanosine 5-monophosphate reductase regulates the enzymatic activity in response to guanylate nucleotide levels. Recombinant enzyme LmGMPR complements the DELTAguaC mutation in Escherichia coli strain H1174 lacking bacterial GMPR | Leishmania major |