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Literature summary for 1.7.1.7 extracted from

  • Wawrzyniak, J.A.; Bianchi-Smiraglia, A.; Bshara, W.; Mannava, S.; Ackroyd, J.; Bagati, A.; Omilian, A.R.; Im, M.; Fedtsova, N.; Miecznikowski, J.C.; Moparthy, K.C.; Zucker, S.N.; Zhu, Q.; Kozlova, N.I.; Berman, A.E.; Hoek, K.S.; Gudkov, A.V.; Shewach, D.S.; Morrison, C.D.; Nikiforov, M.A.
    A purine nucleotide biosynthesis enzyme guanosine monophosphate reductase is a suppressor of melanoma invasion (2013), Cell Rep., 5, 493-507 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutant enzymes in SK-Mel-103 melanoma cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
C186A site-directed mutagenesis, a catalytically inactive mutant, overexpression of GMPRC186A mutant at levels comparable to overexpression of wild-type GMPR does not affect invasion in of melanoma cells Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GMP + NADPH + H+ Homo sapiens
-
 IMP + NH3 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P36959
-
-

Source Tissue

Source Tissue Comment Organism Textmining
melanocyte
-
 Homo sapiens
-
melanoma cell expression of guanosine monophosphate reductase (GMPR), an enzyme involved in de novo biosynthesis of purine nucleotides, is downregulated in invasive stages of human melanoma. From 9 arbitrarily chosen human metastatic melanoma cell lines, GMPR is expressed in only one cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GMP + NADPH + H+
-
 Homo sapiens IMP + NH3 + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
GMPR
-
 Homo sapiens
guanosine monophosphate reductase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens expression of guanosine monophosphate reductase (GMPR), an enzyme involved in de novo biosynthesis of purine nucleotides, is downregulated in invasive stages of human melanoma down

General Information

General Information Comment Organism
malfunction expression of guanosine monophosphate reductase (GMPR), an enzyme involved in de novo biosynthesis of purine nucleotides, is downregulated in invasive stages of human melanoma. Overexpression of catalytically inactive mutant GMPRC186A at levels comparable to overexpression of wild-type GMPR does not affect invasion in of melanoma cells Homo sapiens
metabolism the enzyme activity establishes a link between guanosine metabolism and RHOGTPase-dependent melanoma cell invasion Homo sapiens
physiological function GMPR downregulates the amounts of several GTP-bound (active) RHO-GTPases, and suppresses the ability of melanoma cells to form invadopodia, to degrade extracellular matrix and invade in vitro, and to grow as tumor xenografts in vivo. Enzyme GMPR partially depletes intracellular GTP pools. GMPR is a melanoma invasion suppressor, its enzymatic activity affects melanoma cell invasion and melanoma cell tumorigenicity. GMPR affects formation of invadopodia and matrix degradation. GMPR differentially regulates activity of several RHO-family GTPases, overview Homo sapiens