Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 as His-tag fusion protein | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + guanosine 5'-phosphate | Homo sapiens | essential for the salvage pathway of purine ribonucleotide biosynthesis | NADP+ + inosine 5'-phosphate + NH3 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme using His-tag | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + guanosine 5'-phosphate | - |
Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
ir | |
NADPH + guanosine 5'-phosphate | essential for the salvage pathway of purine ribonucleotide biosynthesis | Homo sapiens | NADP+ + inosine 5'-phosphate + NH3 | - |
ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |