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Literature summary for 1.7.1.6 extracted from
- Accelerated production of oxygen-insensitive azoreductase from mutant Pseudomonas species for degradation azo dyes under aerobic condition (2016), Asian J. Chem., 28, 2562-2570 .
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
naphthol blue black | pH 7.0, 30°C | Pseudomonas sp. B1 |  |
| 0.09 | - |
NADH | pH 7.0, 30°C | Pseudomonas sp. B1 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Pseudomonas sp. B1 | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
native PAGE | Pseudomonas sp. B1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. B1 | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen insensitive intracellular azoreductase | Pseudomonas sp. B1 |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native intracellular enzyme 94.2fold from mutant strain B1 by ammonium sulfate fracionation and anion exchange chromatography | Pseudomonas sp. B1 |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | maximal enzyme production of 0.39 U/ml in mutant strain B1 at pH 6.5, 35°C, 2% D-glucose, 1% sodium nitrate, and 2% of inoculum concentration | Pseudomonas sp. B1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.8 | - |
purified native enzyme, pH 7.0, 30°C | Pseudomonas sp. B1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| naphthol blue black + NADH + H+ | 78% conversion, analysis by UV-visible and FITR spectroscopy, HPLC, and GC-MS | Pseudomonas sp. B1 | ? + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 29000, SDS-PAGE | Pseudomonas sp. B1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| azoreductase | - |
Pseudomonas sp. B1 |
| oxygen insensitive intracellular azoreductase | - |
Pseudomonas sp. B1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pseudomonas sp. B1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | activity range, profile overview | Pseudomonas sp. B1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Pseudomonas sp. B1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | activity range, profile overview | Pseudomonas sp. B1 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Pseudomonas sp. B1 | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme degrades the azo dye naphthol blue black, the reaction product is less toxic than the substrate | Pseudomonas sp. B1 |
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