Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
45000 | - |
gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) B29 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.4 | - |
cofactor FAD, pH not specified in the publication, temperature not specified in the publication | Bacillus sp. (in: Bacteria) |
26.9 | - |
cofactor FMN, pH not specified in the publication, temperature not specified in the publication | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxidized methyl red + NADH + H+ | - |
Bacillus sp. (in: Bacteria) | reduced methyl red + NAD+ | - |
? | |
oxidized methyl red + NADH + H+ | - |
Bacillus sp. (in: Bacteria) B29 | reduced methyl red + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms | Comment | Organism |
---|---|---|
AzrA | - |
Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | about 60% of the activity in presence of FMN. FAD binds to the apo-form and the bound FAD is stably retained in the enzyme molecule without degradation to FMN. The isoalloxazine ring of FAD localizes at the same site and plays the same role as that of FMN in the enzyme | Bacillus sp. (in: Bacteria) | |
FMN | - |
Bacillus sp. (in: Bacteria) |