Application | Comment | Organism |
---|---|---|
environmental protection | potential for the treatment of azo dye contaminated wastewater | Pantoea agglomerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | IC50: 0.13 mM | Pantoea agglomerans | |
Fe2+ | IC50: 0.11 mM | Pantoea agglomerans | |
Hg2+ | IC50: 0.17 mM | Pantoea agglomerans | |
Mn2+ | IC50: 0.43 mM | Pantoea agglomerans | |
Zn2+ | IC50: 0.28 mM | Pantoea agglomerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
2-[4-(dimethylamino)phenylazo]benzoic acid | pH 7.0, 25°C | Pantoea agglomerans | |
0.059 | - |
NADH | pH 7.0, 25°C | Pantoea agglomerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
native PAGE | Pantoea agglomerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pantoea agglomerans |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol | Pantoea agglomerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-[4-(dimethylamino)phenylazo]benzoic acid + NADH | substrate is Methyl red | Pantoea agglomerans | 2-aminobenzoic acid + N,N-dimethyl-1,4-phenylenediamine + NAD+ | - |
? | |
Amaranth + NADH | 35% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? | |
Disperse yellow + NADH | 85% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? | |
Orange G + NADH | 30% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? | |
Trypan blue + NADH | 50% of the activity with Methyl red | Pantoea agglomerans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Pantoea agglomerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pantoea agglomerans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | - |
Pantoea agglomerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pantoea agglomerans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | - |
Pantoea agglomerans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pantoea agglomerans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.11 | - |
IC50: 0.11 mM | Pantoea agglomerans | Fe2+ | |
0.13 | - |
IC50: 0.13 mM | Pantoea agglomerans | Cd2+ | |
0.17 | - |
IC50: 0.17 mM | Pantoea agglomerans | Hg2+ | |
0.28 | - |
IC50: 0.28 mM | Pantoea agglomerans | Zn2+ | |
0.43 | - |
IC50: 0.43 mM | Pantoea agglomerans | Mn2+ |