Application | Comment | Organism |
---|---|---|
environmental protection | potential for the treatment of azo dye contaminated wastewater | Xenophilus azovorans |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS using a phage T7-promotor system, no in vivo activity in recombinant strains due to missing azo dye uptake system | Xenophilus azovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
gel filtration, differences in molecular weights are supposed to be due to an extraordinary behavior of the enzyme in gel filtration | Xenophilus azovorans |
30000 | - |
SDS-PAGE | Xenophilus azovorans |
30280 | - |
calculated from the deduced amino acid sequence | Xenophilus azovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | Xenophilus azovorans | substrate can be utilized as sole carbon and energy source | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | Xenophilus azovorans KF46F / DSM 13620 | substrate can be utilized as sole carbon and energy source | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenophilus azovorans | Q8KU07 | formerly Pseudomonas sp. KF46F | - |
Xenophilus azovorans KF46F / DSM 13620 | Q8KU07 | formerly Pseudomonas sp. KF46F | - |
Purification (Comment) | Organism |
---|---|
- |
Xenophilus azovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.8 | - |
purified enzyme, pH 7.1 with 1-(4'-carboxyphenylazo)-2-naphthol and NADH as substrates | Xenophilus azovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | substrate can be utilized as sole carbon and energy source | Xenophilus azovorans | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | substrate is carboxy-Orange II | Xenophilus azovorans | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | substrate can be utilized as sole carbon and energy source | Xenophilus azovorans KF46F / DSM 13620 | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-2-naphthol + NAD(P)H | substrate is carboxy-Orange II | Xenophilus azovorans KF46F / DSM 13620 | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-2-hydroxynaphthalene | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Xenophilus azovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | flavin-free enzyme | Xenophilus azovorans | |
NADH | - |
Xenophilus azovorans | |
NADPH | preferred over NADH | Xenophilus azovorans |