Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Orange I | substrate inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
NADPH | - |
Pseudomonas sp. | |
0.0024 | - |
1-(4'-carboxyphenylazo)-4-naphthol | - |
Pseudomonas sp. | |
0.0026 | - |
1-(4'-sulfophenylazo)-4-naphthol | - |
Pseudomonas sp. | |
0.04 | - |
1-(4'-sulfophenyl-N-methylhydrazo)-4-naphthol | - |
Pseudomonas sp. | |
0.054 | - |
NADH | - |
Pseudomonas sp. | |
0.204 | - |
1-(4'-sulfophenylazo)-4-anthranol | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20200 | - |
1 * 20200, strain K24, SDS-PAGE, Orange I azoreductase | Pseudomonas sp. |
21500 | - |
strain KF 24, gel filtration, Orange I azoreductase | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(dimethylamino)azobenzene + 2 NADPH | Pseudomonas sp. | key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | Pseudomonas sp. K24 | key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. K24 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.87 | - |
- |
Pseudomonas sp. |
2868 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H | - |
Pseudomonas sp. | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene | - |
? | |
1-(4'-carboxyphenylazo)-4-naphthol + NAD(P)H | - |
Pseudomonas sp. K24 | 4-amino-1-benzoic acid + NAD(P)+ + 1-amino-4-hydroxynaphthalene | - |
? | |
1-(4'-sulfophenyl-N-methylhydrazo)-4-naphthol + NADH + H+ | i.e. carboxy-Orange I | Pseudomonas sp. | ? | - |
r | |
1-(4'-sulfophenylazo)-4-anthranol + NADH + H+ | - |
Pseudomonas sp. | ? | - |
r | |
1-(4'-sulfophenylazo)-4-naphthol + NADH + H+ | - |
Pseudomonas sp. | ? | - |
r | |
4-(dimethylamino)azobenzene + 2 NADPH | Orange I azoreductase: absolute requirement for a hydroxyl group in 4'-position of the naphthol ring of the substrate molecule | Pseudomonas sp. | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | Orange II azoreductase: requirement for substrates with a 2-naphthol moiety | Pseudomonas sp. | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures | Pseudomonas sp. | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | Orange I azoreductase: absolute requirement for a hydroxyl group in 4'-position of the naphthol ring of the substrate molecule | Pseudomonas sp. K24 | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | Orange II azoreductase: requirement for substrates with a 2-naphthol moiety | Pseudomonas sp. K24 | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-(dimethylamino)azobenzene + 2 NADPH | key enzyme of azo dye degradation, selective agent during experimental evolution in continuous cultures | Pseudomonas sp. K24 | N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ + 2 H+ | - |
? | |
4-[(4-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H | trivial name Orange I | Pseudomonas sp. | 4-amino-benzenesulfonic acid + 1-amino-4-hydroxynaphthalene + NADP+ | - |
? | |
4-[(4-hydroxy-1-naphthalenyl)azo]benzene sulfonic acid + NAD(P)H | trivial name Orange I | Pseudomonas sp. K24 | 4-amino-benzenesulfonic acid + 1-amino-4-hydroxynaphthalene + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 20200, strain K24, SDS-PAGE, Orange I azoreductase | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
Orange I azoreductase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
41 | - |
- |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
Orange I | Pseudomonas sp. |
6.8 | - |
carboxy-Orange I | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas sp. | |
NADPH | - |
Pseudomonas sp. |