BRENDA - Enzyme Database show
show all sequences of 1.7.1.4

Purification and properties of nitrite reductase from Escherichia coli K12

Coleman, K.J.; Cornish-Bowden, A.; Cole, J.A.; Biochem. J. 175, 483-493 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Escherichia coli
2,2'-bipyridine
-
Escherichia coli
arsenite
10 mM, 24% inhibition
Escherichia coli
CN-
-
Escherichia coli
NaN3
-
Escherichia coli
p-chloromercuribenzoate
0.02 mM, complete inactivation, partially prevented by 10 mM cysteine
Escherichia coli
SO32-
1 mM, 22% inhibition
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell associated
-
Escherichia coli
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
88000
-
2 * 88000, SDS-PAGE
Escherichia coli
190000
-
glycerol density gradient centrifugation
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD(P)H + nitrite + H+
Escherichia coli
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli
?
NAD(P)H + nitrite + H+
Escherichia coli OR75Ch15
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli OR75Ch15
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli OR75Ch15
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
16.7
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD(P)H + NH2OH
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
NAD(P)H + NH2OH
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
NAD(P)H + nitrite + H+
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
NAD(P)H + nitrite + H+
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 88000, SDS-PAGE
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
flavin
0.4 mol of flavin per mol of enzyme
Escherichia coli
additional information
heme is not detectable
Escherichia coli
NADH
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
flavin
0.4 mol of flavin per mol of enzyme
Escherichia coli
additional information
heme is not detectable
Escherichia coli
NADH
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Escherichia coli
2,2'-bipyridine
-
Escherichia coli
arsenite
10 mM, 24% inhibition
Escherichia coli
CN-
-
Escherichia coli
NaN3
-
Escherichia coli
p-chloromercuribenzoate
0.02 mM, complete inactivation, partially prevented by 10 mM cysteine
Escherichia coli
SO32-
1 mM, 22% inhibition
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cell associated
-
Escherichia coli
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
88000
-
2 * 88000, SDS-PAGE
Escherichia coli
190000
-
glycerol density gradient centrifugation
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
NAD(P)H + nitrite + H+
Escherichia coli
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli
?
NAD(P)H + nitrite + H+
Escherichia coli OR75Ch15
-
NAD(P)+ + NH3 + H2O
-
Escherichia coli OR75Ch15
?
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
16.7
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD(P)H + NH2OH
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
NAD(P)H + NH2OH
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
NAD(P)H + nitrite + H+
-
392923
Escherichia coli
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli
?
NAD(P)H + nitrite + H+
-
392923
Escherichia coli OR75Ch15
NAD(P)+ + NH3 + H2O
-
392923
Escherichia coli OR75Ch15
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 88000, SDS-PAGE
Escherichia coli
Other publictions for EC 1.7.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673745
Garbeva
Phylogeny of nitrite reductase ...
Nitrosomonas europaea, Nitrosospira briensis 128, Nitrosospira briensis, Nitrosospira multiformis, Nitrosospira sp., Nitrosospira tenuis
FEMS Microbiol. Lett.
266
83-89
2007
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676081
Pittman
Growth of Campylobacter jejuni ...
Campylobacter jejuni, Campylobacter jejuni NCTC 11168
Mol. Microbiol.
63
575-590
2007
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1
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14
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4
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674181
Usov
EPR-ENDOR of the Cu(I)NO Compl ...
Rhodobacter sphaeroides
J. Am. Chem. Soc.
128
13102-13111
2006
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2
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671935
Bueno
Function of the Rhizobium etli ...
Rhizobium etli, Rhizobium etli CFN 42
Biochem. Soc. Trans.
33
162-163
2005
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1
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1
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7
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2
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2
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2
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674214
Sar
Effect of Escherichia coli wil ...
Escherichia coli, Escherichia coli W3110 / ATCC 27325
J. Anim. Sci.
83
644-652
2005
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1
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27
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2
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675640
Nathawat
-
Nitrogen-metabolizing enzymes: ...
Brassica juncea
J. Plant Nutr.
28
1089-1101
2005
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1
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655284
Jargeat
Characterisation and expressio ...
Hebeloma cylindrosporum
Curr. Genet.
43
199-205
2003
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1
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655625
Polcyn
Aerobic and anaerobic nitrate ...
Bradyrhizobium sp.
FEMS Microbiol. Lett.
226
331-337
2003
1
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3
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1
4
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1
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1
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1
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392944
Nakano
Nitrogen and oxygen regulation ...
Bacillus subtilis
J. Bacteriol.
180
5344-5350
1998
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2
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392941
Tyson
Catabolite regulation of two E ...
Escherichia coli
Arch. Microbiol.
168
240-244
1997
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1
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392942
Sengupta
In vitro and in vivo regulatio ...
Cyberlindnera jadinii
Arch. Microbiol.
168
215-224
1997
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3
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392943
McNally
A high-throughput assay format ...
Cyberlindnera jadinii
Appl. Biochem. Biotechnol.
62
29-36
1997
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392940
Neubauer
Physiology and interaction of ...
Staphylococcus carnosus
J. Bacteriol.
178
2005-2009
1996
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2
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392937
Wang
-
Some properties of nitrite and ...
Derxia gummosa
Phytochemistry
25
2463-2469
1986
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15
4
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2
4
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392924
MacDonald
Molecular cloning and function ...
Escherichia coli
Mol. Gen. Genet.
200
328-334
1985
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392925
Cammack
Electron-spin-resonance studie ...
Escherichia coli
Biochem. J.
207
333-339
1982
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392926
Jackson
The steady-state kinetics of t ...
Escherichia coli
Biochem. J.
199
171-178
1981
1
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392930
Prodouz
Neurospora crassa NAD(P)H-nitr ...
Neurospora crassa
J. Biol. Chem.
256
9711-9717
1981
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3
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2
1
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1
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4
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392923
Coleman
Purification and properties of ...
Escherichia coli, Escherichia coli OR75Ch15
Biochem. J.
175
483-493
1978
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7
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1
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2
2
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4
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1
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1
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4
1
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3
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3
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7
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1
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2
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1
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392928
Coleman
Activation of nitrite reductas ...
Escherichia coli
Biochem. J.
175
495-499
1978
1
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1
3
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392931
Greenbaum
Preparation and some propertie ...
Neurospora crassa
Biochim. Biophys. Acta
526
52-64
1978
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6
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2
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1
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1
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1
1
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2
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1
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287801
Hatefi
-
Metal-containing flavoprotein ...
Torulopsis nitratophila
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
13
175-297
1976
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2
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1
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1
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392932
Vega
Siroheme: a prosthetic group o ...
Neurospora crassa
J. Biol. Chem.
250
7980-7989
1975
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3
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1
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392933
Vega
Studies on the in vitro inacti ...
Neurospora crassa
Biochim. Biophys. Acta
377
251-257
1975
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1
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1
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1
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1
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1
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392934
Lafferty
Purification and properties of ...
Neurospora crassa
J. Biol. Chem.
249
7555-7567
1974
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8
4
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1
1
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1
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1
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2
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2
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1
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1
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2
6
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2
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8
6
4
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1
1
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1
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2
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2
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1
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1
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392936
Vega
Reduced nicotinamide-adenine d ...
Azotobacter chroococcum
Biochem. J.
133
701-708
1973
1
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7
2
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1
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1
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1
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4
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7
1
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1
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392929
Lazzarini
A triphosphopyridine nucleotid ...
Escherichia coli, Escherichia coli Bn
J. Biol. Chem.
236
3330-3335
1961
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-
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1
4
1
1
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2
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1
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2
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1
1
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392935
Nicholas
A nitrite reductase from Neuro ...
Neurospora crassa
Biochim. Biophys. Acta
37
468-476
1960
2
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1
15
3
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4
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1
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1
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1
1
1
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1
1
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2
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2
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1
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15
-
3
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4
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1
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1
1
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