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Literature summary for 1.7.1.3 extracted from
- Biochemical characterization of molybdenum cofactor-free nitrate reductase from Neurospora crassa (2013), J. Biol. Chem., 288, 14657-14671 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| sequence comparisons, recombinant enzyme expression of wild-type and mutant enzymes in Escherichia coli strains TP1000, RK5206, and RK5204 | Neurospora crassa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G811V | site-directed mutagenesis, an FAD-binding mutant | Neurospora crassa |
| H654A/H677A | site-directed mutagenesis, CD spectroscopy shows no negative effects of the introduced mutations on protein secondary structure in comparison to the wild-type protein, but the mutant contains no heme, while the FAD binding ability is not significantly disturbed | Neurospora crassa |
| R778E | site-directed mutagenesis, an FAD-binding mutant. The mutant binds essentially the same amount of Moco as does the wild type protein | Neurospora crassa |
| Y780A | site-directed mutagenesis, an FAD-binding mutant | Neurospora crassa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics, holo-enzyme | Neurospora crassa | |
| 0.25 | - |
nitrate | recombinant holo-enzyme, pH 7.5, temperature not specified in the publication | Neurospora crassa |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | heme | Neurospora crassa | |
| Mo5+ | in the active site-bound molybdenum cofactor | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
gel filtration and analytical ultracentrifugation | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH + H+ | Neurospora crassa | - |
nitrite + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strains TP1000, RK5206, and RK5204 by a two-step affinity purification | Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.32 | - |
purified recombinant holo-enzyme, substrate nitrate, pH 7.5, temperature not specified in the publication | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | NADPH-dependent cytochrome c reducing activity by the holo-enzyme is determined with FAD and NADPH spectroscopically at 550 nm and 340 nm. Apo-nitrate reductase has a marginally lower, about 10% reduced cytochrome c reducing activity, which correlates to its 15% reduced heme content | Neurospora crassa | ? | - |
? | |
| nitrate + NADPH + H+ | - |
Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | holo-enzyme, determination with SDS-PAGE, gel filtration and analytical ultracentrifugation. The enzyme has a largely hydrophobic dimer interface | Neurospora crassa |
| More | the apo-enzyme dissociates completely into monomers, the ratio between monomeric and dimeric apo-NR does not change significantly upon a 20fold dilution. Active site formation of eukaryotic nitrate reductase is an autonomous process intrinsically tied to nitrate reductase dimerization, molybdenum cofactor-dependent enzyme maturation, overview. Enzyme domain structure, overview | Neurospora crassa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrate reductase [NADPH] | - |
Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | - |
Neurospora crassa | |
| FAD | the enzyme contains a a ferredoxin reductase-type FAD binding domain | Neurospora crassa | |
| heme | enzyme heme binding is independent from Moco/MPT binding | Neurospora crassa | |
| molybdenum cofactor | i.e Moco/MPT, binding of molybdenum cofactor to apo-nitrate reductase is independent from other prosthetic groups, molybdenum cofactor-dependent enzyme maturation, overview. Reconstitution of Moco-free nitrate reductase with various amounts of purified Moco carrier protein | Neurospora crassa | |
| molybdopterin | i.e Moco/MPT, binding of molybdenum cofactor to apo-nitrate reductase is independent from other prosthetic groups, molybdenum cofactor-dependent enzyme maturation, overview. Reconstitution of Moco-free nitrate reductase with various amounts of purified Moco carrier protein | Neurospora crassa | |
| NADPH | dependent on | Neurospora crassa | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | active site formation of eukaryotic nitrate reductase is an autonomous process intrinsically tied to nitrate reductase dimerization, molybdenum cofactor-dependent enzyme maturation, overview | Neurospora crassa |
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