Any feedback?
Literature summary for 1.7.1.3 extracted from
- Preparation and proterties of apoenzyme of nitrate reductases from wild-type and nit-3 mutant of Neurospora crassa (1983), Biochim. Biophys. Acta, 744, 16-22.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme can be reactivated by molybdenum and dithioerythritol | Neurospora crassa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | nit-1 mutant: lacks all activities except FAD-dependent NADPH:cytochrome c reductase activity,nit-2 mutant: reduced FAD:- and reduced methyl viologen:nitrate reductase activities but lacks the other two activities | Neurospora crassa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | 1 mol per mol enzyme protein, activation of enzyme after urea treatment, little stimulation | Neurospora crassa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 132000 | - |
2 * 97000: nit-3 enzyme, 2 * 132000, wild-type | Neurospora crassa |
| 204000 | - |
nit-3 enzyme, gel filtration | Neurospora crassa |
| 272000 | - |
wild-type, gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | Neurospora crassa | first step in nitrate assimilation | nitrite + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
wild-type STA4, nit-1 and nit-3 mutants | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially purification of wild-type and nit-3 mutant by streptomycin sulfate precipitation and ammonium sulfate fractionation, of nit-1 mutant by protamine sulfate fractionation, ammonium sulfate precipitation and gel filtration | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, urea-treated, 0.1 M potassium phosphate buffer, pH 7.3, 5 mM EDTA, 2 mM dithioerythritol, 0.5 mg/ml or more bovine serum albumin, stable for 18 h without loss of activity | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | first step in nitrate assimilation | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 97000: nit-3 enzyme, 2 * 132000, wild-type | Neurospora crassa |
| More | nit-1 mutant enzyme is the apoprotein of nitrate reductase | Neurospora crassa |
| More | aggregate of two different polypeptide chains: one responsible for transport of electrons fom NADPH to FAD or cytochrome c, nit-1 enzyme. The other transfers electrons from FAD via molybdenum to nitrate, nit-3 enzyme | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b557 | - |
Neurospora crassa | |
| heme | 2 mol heme iron per mol enzyme protein | Neurospora crassa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
