Activating Compound | Comment | Organism | Structure |
---|---|---|---|
o-phenanthroline | 40% increase of activity at 2.5 mM | Neurospora crassa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 94% inhibition at 10 mM | Neurospora crassa | |
azide | 96% inhibition at 5 mM | Neurospora crassa | |
cyanide | - |
Neurospora crassa | |
EDTA | - |
Neurospora crassa | |
additional information | protection of inactivation by FAD and restorage by dithiothreitol | Neurospora crassa | |
p-hydroxymercuribenzoate | reversed by sulfhydryl reagents | Neurospora crassa | |
Phenylglyoxal | in 0.1 M phosphate, pH 7.3, 4 mM, inactivation after 15 min to 40% and to 20% after 60 min | Neurospora crassa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Molybdenum | 1 mol per mol protein | Neurospora crassa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
130000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
150000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
230000 | - |
sucrose density gradient, gel filtration | Neurospora crassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | Neurospora crassa | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | Neurospora crassa STA4 | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | nitrite + NADP+ + H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | mild denaturation | Neurospora crassa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neurospora crassa | - |
STA4 | - |
Neurospora crassa STA4 | - |
STA4 | - |
Purification (Comment) | Organism |
---|---|
streptomycin sulfate and ammonium sulfate precipitation, ion-exchange, gel filtration, isoelectric focusing, FAD-affinity | Neurospora crassa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Neurospora crassa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Neurospora crassa |
Storage Stability | Organism |
---|---|
-10°C, 50 mM sodium phosphate, pH 6.9, 30% glycerol, 0.5 mM EDTA, 0.5 mM dithiothreitol, 0.5 mM phenylmethylsulfonylfluoride, 0.1 mM FAD, stable for at least 6 months | Neurospora crassa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa STA4 | nitrite + NADP+ + H2O | - |
? | |
nitrate + NADPH | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | Neurospora crassa STA4 | nitrite + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome b557 | heme-iron, 2 mol per mol enzyme | Neurospora crassa | |
FAD | 1 mol per subunit is assumed | Neurospora crassa |