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Literature summary for 1.7.1.3 extracted from
- Purification and characterization of homogeneous assimilatory reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase from Neurospora crassa (1978), Biochim. Biophys. Acta, 523, 297-313.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| o-phenanthroline | 40% increase of activity at 2.5 mM | Neurospora crassa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 8-hydroxyquinoline | 94% inhibition at 10 mM | Neurospora crassa | |
| azide | 96% inhibition at 5 mM | Neurospora crassa | |
| cyanide | - |
Neurospora crassa | |
| EDTA | - |
Neurospora crassa | |
| additional information | protection of inactivation by FAD and restorage by dithiothreitol | Neurospora crassa | |
| p-hydroxymercuribenzoate | reversed by sulfhydryl reagents | Neurospora crassa | |
| Phenylglyoxal | in 0.1 M phosphate, pH 7.3, 4 mM, inactivation after 15 min to 40% and to 20% after 60 min | Neurospora crassa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | 1 mol per mol protein | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 115000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
| 130000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
| 150000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
| 230000 | - |
sucrose density gradient, gel filtration | Neurospora crassa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | Neurospora crassa | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | Neurospora crassa STA4 | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | nitrite + NADP+ + H2O | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | mild denaturation | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
STA4 | - |
| Neurospora crassa STA4 | - |
STA4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| streptomycin sulfate and ammonium sulfate precipitation, ion-exchange, gel filtration, isoelectric focusing, FAD-affinity | Neurospora crassa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Neurospora crassa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, 50 mM sodium phosphate, pH 6.9, 30% glycerol, 0.5 mM EDTA, 0.5 mM dithiothreitol, 0.5 mM phenylmethylsulfonylfluoride, 0.1 mM FAD, stable for at least 6 months | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa STA4 | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | Neurospora crassa STA4 | nitrite + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome b557 | heme-iron, 2 mol per mol enzyme | Neurospora crassa | |
| FAD | 1 mol per subunit is assumed | Neurospora crassa | |
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