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Literature summary for 1.7.1.3 extracted from
- Studies on temperature-sensitive mutants affecting the assimilatory nitrate reductase of Aspergillus nidulans (1974), Eur. J. Biochem., 47, 107-110.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | studies of temperature-sensitive mutations, niaD gene: mutation leads to loss of a 4.5-S cytochrome-c reductase activity, which is a subunit of nitrate reductase. It is suggested that neither the product of the cnxE nor the cnyF genes form part of the nitrate reductase molecule, but some catalytic role in cofactor formation, niaD and cnxH seem to be structural genes | Aspergillus nidulans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | molybdenum containing cofactor | Aspergillus nidulans |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | Aspergillus nidulans | - |
nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | Aspergillus nidulans biA-1 | - |
nitrite + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
biA-1 | - |
| Aspergillus nidulans biA-1 | - |
biA-1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.004 | 0.074 | various temperature-sensitive mutants | Aspergillus nidulans |
| 0.074 | - |
wild type | Aspergillus nidulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrate + NADPH | - |
Aspergillus nidulans | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | some mutants use hypoxanthine as nitrogen source, 4.5-S cytochrome-c reductase activity | Aspergillus nidulans | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | - |
Aspergillus nidulans biA-1 | nitrite + NADP+ + H2O | - |
? | |
| nitrate + NADPH | some mutants use hypoxanthine as nitrogen source, 4.5-S cytochrome-c reductase activity | Aspergillus nidulans biA-1 | nitrite + NADP+ + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| multimer | - |
Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
wild-type half-life: 120 min | Aspergillus nidulans |
| 30 | - |
wild-type half-life: 66 min | Aspergillus nidulans |
| 35 | - |
wild-type half-life: 18.8 min, various temperature sensitive mutant strains: 3.8-18.7 min | Aspergillus nidulans |
| 37 | - |
wild-type half-life: 14.5 min | Aspergillus nidulans |
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Release 2023.1 (January 2023)
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