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Literature summary for 1.7.1.2 extracted from

  • Sempruch, C.; Ciepiela, A.; Sprawka, I.; Chrzanowski, G.
    Purification and some physiochemical properties of nitrate reductase isolated from winter triticale seedlings (2008), Electron. J. Pol. Agric. Univ., 11, 1-1.
No PubMed abstract available
Search for more literature for 1.7.1.2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
 SDS-PAGE Triticale sp.

Organism

Organism UniProt Comment Textmining
Triticale sp.
-
 Triticum sativum x Secale cereale, winter triticale
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and blue-dextran-Sepharose column chromatography Triticale sp.

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Triticale sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000013
-
 crude extract, using FMNH as electron donor, in 50 mM Tris-HCl buffer, pH 7.4, at 25°C Triticale sp.
0.0000167
-
 crude extract, using methyl viologen as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.7, at 25°C Triticale sp.
0.000117
-
 crude extract, using NADPH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25°C Triticale sp.
0.000184
-
 crude extract, using NADH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25°C Triticale sp.
0.455
-
 after 2481.9fold purification, using NADH as electron donor, in 0.1 mM potassium phosphate buffer, pH 7.5, at 25°C Triticale sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD(P)H + H+ + nitrate
-
 Triticale sp. NAD(P)+ + nitrite + H2O
-
 ?
reduced methyl viologen + nitrate
-
 Triticale sp. oxidized methyl viologen + nitrite + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 190000, SDS-PAGE Triticale sp.

Cofactor

Cofactor Comment Organism Structure
NAD(P)H the enzyme is specific in relation to NADH as well as to NADPH although it shows lower specific activity in the presence of NADPH Triticale sp.