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Literature summary for 1.7.1.17 extracted from

  • Chen, H.; Wang, R.; Cerniglia, C.
    Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis (2004), Protein Expr. Purif., 34, 302-310 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.7.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid pH 7.1, 23°C Enterococcus faecalis
0.14
-
 NADH pH 7.1, 23°C Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 gel filtration Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q831B2
-
-
Enterococcus faecalis ATCC 19433 Q831B2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
137
-
 substrate amaranth, pH 7.1, 23°C Enterococcus faecalis
139
-
 substrate orange II, pH 7.1, 23°C Enterococcus faecalis
167
-
 substrate ponceau S, pH 7.1, 23°C Enterococcus faecalis
181
-
 substrate 4'-(dimethylamino)-azobenzene-2-carboxylic acid, pH 7.1, 23°C Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+
-
 ?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 19433 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+
-
 ?
amaranth + 2 NADH + 2 H+
-
 Enterococcus faecalis 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+
-
 ?
amaranth + 2 NADH + 2 H+
-
 Enterococcus faecalis ATCC 19433 4-aminonaphthalene-1-sulfonic acid + 4-amino-3-hydroxynaphthalene-2,7-disulfonic acid + 2 NAD+
-
 ?
orange G + 2 NADPH + 2 H+
-
 Enterococcus faecalis aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+
-
 ?
orange G + 2 NADPH + 2 H+
-
 Enterococcus faecalis ATCC 19433 aniline + 8-amino-7-hydroxynaphthalene-1,3-disulfonic acid + 2 NADP+
-
 ?
orange II + 2 NADPH + 2 H+
-
 Enterococcus faecalis 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NADP+ + 2 NAD+
-
 ?
orange II + 2 NADPH + 2 H+
-
 Enterococcus faecalis ATCC 19433 4-aminobenzene-1-sulfonic acid + 1-aminonaphthalen-2-ol + 2 NADP+ + 2 NAD+
-
 ?
ponceau BS + 2 NADH + 2 H+
-
 Enterococcus faecalis ? + 2 NAD+
-
 ?
ponceau BS + 2 NADH + 2 H+
-
 Enterococcus faecalis ATCC 19433 ? + 2 NAD+
-
 ?
ponceau S + 2 NADH + 2 H+
-
 Enterococcus faecalis ? + 2 NAD+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE Enterococcus faecalis

Synonyms

Synonyms Comment Organism
AzoA
-
 Enterococcus faecalis

Cofactor

Cofactor Comment Organism Structure
FMN one molecule of FMN per dimer Enterococcus faecalis
additional information no cofactor: NADPH Enterococcus faecalis
NADH
-
 Enterococcus faecalis