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Literature summary for 1.7.1.17 extracted from

  • Punj, S.; John, G.
    Purification and identification of an FMN-dependent NAD(P)H azoreductase from Enterococcus faecalis (2009), Curr. Issues Mol. Biol., 11, 59-66 .
    View publication on PubMed
Search for more literature for 1.7.1.17

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid pH 7.2, 25°C Enterococcus faecalis
0.082
-
 NADH pH 7.2, 25°C Enterococcus faecalis
15
-
 NADPH pH 7.2, 25°C Enterococcus faecalis

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-
Enterococcus faecalis ATCC 27274
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.626
-
 cofactor NADPH, pH 7.0, 25°C Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 27274 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+
-
 ?
4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADPH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 27274 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NADP+
-
 ?

Cofactor

Cofactor Comment Organism Structure
FMN Km value 0.003 mM Enterococcus faecalis
NADH 180fold preferred over NADPH Enterococcus faecalis
NADPH NADH is 180fold preferred over NADPH Enterococcus faecalis