BRENDA - Reference to 1.7.1.16; Id = 741093

Salmonella enterica subsp. enterica serovar Typhimurium

P15888

-

Salmonella enterica subsp. enterica serovar Typhimurium LT2

P15888

-

8.1

-

substrate nitrofurazone, pH 7.2, 20°C

Salmonella enterica subsp. enterica serovar Typhimurium

13.6

-

substrate nitrofurazone, pH 7.5, 45°C

Salmonella enterica subsp. enterica serovar Typhimurium

additional information

enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%

741093

Salmonella enterica subsp. enterica serovar Typhimurium

?

-

additional information

enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%

741093

Salmonella enterica subsp. enterica serovar Typhimurium LT2

?

-

nitrobenzene + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium

aniline + 2 NADP+ + H2O

reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion

-

?

nitrobenzene + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium LT2

aniline + 2 NADP+ + H2O

reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion

-

?

nitrofurazone + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium

? + 2 NADP+ + H2O

-

?

nitrofurazone + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium LT2

? + 2 NADP+ + H2O

-

?

Oxygen-insensitive NAD(P)H nitroreductase

-

Salmonella enterica subsp. enterica serovar Typhimurium

37

-

half-life 365 h

Salmonella enterica subsp. enterica serovar Typhimurium

45

-

half-life 17.9 h

Salmonella enterica subsp. enterica serovar Typhimurium

50

-

half-life 2.3 h

Salmonella enterica subsp. enterica serovar Typhimurium

7.2

-

Salmonella enterica subsp. enterica serovar Typhimurium

8

-

considerable loss of activity above

Salmonella enterica subsp. enterica serovar Typhimurium

8.1

-

substrate nitrofurazone, pH 7.2, 20°C

Salmonella enterica subsp. enterica serovar Typhimurium

13.6

-

substrate nitrofurazone, pH 7.5, 45°C

Salmonella enterica subsp. enterica serovar Typhimurium

additional information

enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%

741093

Salmonella enterica subsp. enterica serovar Typhimurium

?

-

additional information

enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%

741093

Salmonella enterica subsp. enterica serovar Typhimurium LT2

?

-

nitrobenzene + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium

aniline + 2 NADP+ + H2O

reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion

-

?

nitrobenzene + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium LT2

aniline + 2 NADP+ + H2O

reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion

-

?

nitrofurazone + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium

? + 2 NADP+ + H2O

-

?

nitrofurazone + 2 NADPH + 2 H+

-

741093

Salmonella enterica subsp. enterica serovar Typhimurium LT2

? + 2 NADP+ + H2O

-

?

37

-

half-life 365 h

Salmonella enterica subsp. enterica serovar Typhimurium

45

-

half-life 17.9 h

Salmonella enterica subsp. enterica serovar Typhimurium

50

-

half-life 2.3 h

Salmonella enterica subsp. enterica serovar Typhimurium

7.2

-

Salmonella enterica subsp. enterica serovar Typhimurium

8

-

considerable loss of activity above

Salmonella enterica subsp. enterica serovar Typhimurium

740989

Yang

Heterologous overexpression an ...

Gluconobacter oxydans, Gluconobacter oxydans 621H

Mol. Biotechnol.

58

428-440

2016

-

7

9

-

2

-

1

-

13

-

9

-

1

-

1

-

7

-

9

-

1

-

13

-

9

-

9

740192

Li

In vivo imaging and detection ...

Danio rerio

Biosens. Bioelectron.

63

112-116

2015

-

1

-

1

-

1

-

1

-

1

-

740325

Yuan

-

Isolation and characterization ...

Pseudomonas sp., Pseudomonas sp. N1

Chin. J. Appl. Environ. Biol.

19

1031-1034

2013

-

1

-

2

-

2

-

1

-

2

-

741380

Isayev

In silico structure-function a ...

Enterobacter cloacae

Proteins

80

2728-2741

2012

-

1

-

1

-

1

-

1

-

1

-

741093

Yanto

Nitroreductase from Salmonella ...

Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2

Org. Biomol. Chem.

8

1826-1832

2010

-

2

-

2

-

6

-

1

-

3

-

1

-

2

-

6

-

3

-

1

-

677659

Wu

Novel partial reductive pathwa ...

Comamonas sp., Comamonas sp. Ctestosteroni

Appl. Environ. Microbiol.

72

1759-1765

2006

-

1

-

2

-

2

-

1

-

1

-

1

-

2

-

1

-

677735

Xiao

Characterization of genes invo ...

Pseudomonas putida, Pseudomonas putida ZWL73

Appl. Microbiol. Biotechnol.

73

166-171

2006

-

1

-

7

-

14

-

1

-

2

-

1

2

-

14

-

740133

Berne

Application of a microfluidic ...

Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45

Biomacromolecules

7

2631-2636

2006

-

1

-

6

-

4

-

1

-

6

-

1

-

1

-

6

-

1

-

6

-

740929

Ho

-

Characterization of nitroreduc ...

Pseudomonas sp. HK-6

Korean J. Microbiol. Biotechnol.

32

230-237

2004

-

5

-

1

-

1

-

1

-

1

-

2

1

-

1

-

1

-

5

-

1

-

1

-

1

-

2

1

-

1

-

740596

Somerville

Purification and characterizat ...

Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45

J. Bacteriol.

177

3837-3842

1995

1

-

11

3

-

2

4

-

4

-

1

-

1

-

8

1

-

3

-

1

-

3

-

11

-

3

-

2

4

-

1

-

1

-

8

1

-

739974

Rieble

Aromatic nitroreductase from t ...

Phanerochaete chrysosporium

Biochem. Biophys. Res. Commun.

205

298-304

1994

-

1

-

1

-

1

-

2

-

7

-

1

-

1

-

1

-

1

-

2

-

7

-

1

-

1

-

Nitroreductase from Salmonella typhimurium: characterization and catalytic activity

Data extracted from this reference: