BRENDA - Enzyme Database
show all sequences of 1.7.1.16

Nitroreductase from Salmonella typhimurium: characterization and catalytic activity

Yanto, Y.; Hall, M.; Bommarius, A.S.; Org. Biomol. Chem. 8, 1826-1832 (2010)

Data extracted from this reference:

Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
P15888
-
-
Salmonella enterica subsp. enterica serovar Typhimurium LT2
P15888
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
8.1
-
substrate nitrofurazone, pH 7.2, 20C
Salmonella enterica subsp. enterica serovar Typhimurium
13.6
-
substrate nitrofurazone, pH 7.5, 45C
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%
741093
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%
741093
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
-
-
-
nitrobenzene + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium
aniline + 2 NADP+ + H2O
reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium LT2
aniline + 2 NADP+ + H2O
reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium
? + 2 NADP+ + H2O
-
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium LT2
? + 2 NADP+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Oxygen-insensitive NAD(P)H nitroreductase
-
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
37
-
half-life 365 h
Salmonella enterica subsp. enterica serovar Typhimurium
45
-
half-life 17.9 h
Salmonella enterica subsp. enterica serovar Typhimurium
50
-
half-life 2.3 h
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Range
pH Minimum
pH Maximum
Commentary
Organism
8
-
considerable loss of activity above
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
8.1
-
substrate nitrofurazone, pH 7.2, 20C
Salmonella enterica subsp. enterica serovar Typhimurium
13.6
-
substrate nitrofurazone, pH 7.5, 45C
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%
741093
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
additional information
enzyme shows broad substrate acceptance for various nitro compounds such as 1-nitrocyclohexene and aliphatic nitroalkenes (alkene reductase activity), as well as nitrobenzene (nitroreductase activity), with substrate conversion efficiency of >95%
741093
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
-
-
-
nitrobenzene + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium
aniline + 2 NADP+ + H2O
reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium LT2
aniline + 2 NADP+ + H2O
reduction of nitrobenzene to aniline occurs through the formation of nitrosobenzene and phenylhydroxylamine as intermediates. More than 99% overall conversion
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium
? + 2 NADP+ + H2O
-
-
-
?
nitrofurazone + 2 NADPH + 2 H+
-
741093
Salmonella enterica subsp. enterica serovar Typhimurium LT2
? + 2 NADP+ + H2O
-
-
-
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
37
-
half-life 365 h
Salmonella enterica subsp. enterica serovar Typhimurium
45
-
half-life 17.9 h
Salmonella enterica subsp. enterica serovar Typhimurium
50
-
half-life 2.3 h
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
8
-
considerable loss of activity above
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740989
Yang
Heterologous overexpression an ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Mol. Biotechnol.
58
428-440
2016
-
-
-
-
-
-
7
9
-
-
-
-
-
2
-
-
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1
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13
-
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9
-
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1
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1
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7
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9
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1
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13
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9
-
-
-
-
-
-
-
-
9
9
740192
Li
In vivo imaging and detection ...
Danio rerio
Biosens. Bioelectron.
63
112-116
2015
-
1
1
-
-
-
-
-
-
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1
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1
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1
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1
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740325
Yuan
-
Isolation and characterization ...
Pseudomonas sp., Pseudomonas sp. N1
Chin. J. Appl. Environ. Biol.
19
1031-1034
2013
-
1
1
-
-
-
-
-
-
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2
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2
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1
1
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2
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741380
Isayev
In silico structure-function a ...
Enterobacter cloacae
Proteins
80
2728-2741
2012
-
-
-
1
-
-
-
-
-
-
-
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-
1
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1
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1
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1
1
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-
-
-
-
741093
Yanto
Nitroreductase from Salmonella ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Org. Biomol. Chem.
8
1826-1832
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
6
-
1
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-
3
-
1
1
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-
2
-
6
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
677659
Wu
Novel partial reductive pathwa ...
Comamonas sp., Comamonas sp. Ctestosteroni
Appl. Environ. Microbiol.
72
1759-1765
2006
-
-
1
-
-
-
-
-
-
-
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-
-
2
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2
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1
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1
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1
1
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2
-
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-
1
1
-
-
-
677735
Xiao
Characterization of genes invo ...
Pseudomonas putida, Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
166-171
2006
-
-
1
-
-
-
-
-
-
-
-
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-
7
-
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14
-
1
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2
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1
2
-
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14
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
740133
Berne
Application of a microfluidic ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
Biomacromolecules
7
2631-2636
2006
-
1
1
-
-
-
-
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
6
-
1
-
-
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-
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1
1
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6
-
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1
-
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-
6
-
-
-
-
-
-
-
-
-
-
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-
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740929
Ho
-
Characterization of nitroreduc ...
Pseudomonas sp. HK-6
Korean J. Microbiol. Biotechnol.
32
230-237
2004
-
-
-
-
-
-
5
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
-
1
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-
-
-
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-
-
5
-
-
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-
1
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
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-
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-
-
740596
Somerville
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
1
-
-
-
-
-
11
3
-
-
2
4
-
4
-
-
1
-
-
-
1
-
8
1
-
-
-
-
-
-
-
-
3
-
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-
1
-
-
3
-
-
-
-
11
-
3
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739974
Rieble
Aromatic nitroreductase from t ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
-
-
-
-
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1
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1
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1
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-
-
2
-
7
-
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1
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-
-
1
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-
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-
1
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1
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2
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7
-
1
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1
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