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Literature summary for 1.7.1.16 extracted from

  • Yang, Y.; Lin, J.; Wei, D.
    Heterologous overexpression and biochemical characterization of a nitroreductase from Gluconobacter oxydans 621H (2016), Mol. Biotechnol., 58, 428-440.
    View publication on PubMed
Search for more literature for 1.7.1.16

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 1 mM, no residual activity Gluconobacter oxydans
glutathione 1 mM, 41% residual activity Gluconobacter oxydans
menadione 1 mM, 61% residual activity Gluconobacter oxydans
Sodium azide 1 mM, 52% residual activity Gluconobacter oxydans
Triton X-100 1% w/v, 84% residual activity Gluconobacter oxydans
Tween 20 1% w/v, 64% residual activity Gluconobacter oxydans
ZnSO4 1 mM, no residual activity Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 (2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide pH 8.0, 37°C Gluconobacter oxydans
0.054
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide pH 8.0, 37°C Gluconobacter oxydans
0.128
-
 1,3-dinitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.153
-
 NADPH pH 8.0, 37°C Gluconobacter oxydans
0.264
-
 3-nitrotoluene pH 8.0, 37°C Gluconobacter oxydans
0.282
-
 4-nitrotoluene pH 8.0, 37°C Gluconobacter oxydans
0.309
-
 4-chloronitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.468
-
 2-chloronitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.545
-
 nitrobenzene pH 8.0, 37°C Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q5FSN8
-
-
Gluconobacter oxydans 621H Q5FSN8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11000
-
 substrate nitrobenzene, pH 8.0, 37°C Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+ i.e. anti-infective furacilin Gluconobacter oxydans (2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
-
 ?
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+ i.e. anti-infective furacilin Gluconobacter oxydans 621H (2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
-
 ?
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans ? + 2 NADP+
-
 ?
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans 621H ? + 2 NADP+
-
 ?
2-chloronitrobenzene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans 2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
 ?
2-chloronitrobenzene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans 621H 2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
 ?
3-nitrotoluene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
 ?
3-nitrotoluene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans 621H N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
 ?
4-chloronitrobenzene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans 4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
 ?
4-nitrotoluene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans N-hydroxy-4-methylaniline + 2 NADP+ + H2O
-
 ?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
 Gluconobacter oxydans ? + 2 NADP+ enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide ?
nitrobenzene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans N-hydroxyaniline + 2 NADP+ + H2O
-
 ?
nitrobenzene + 2 NADPH + 2 H+
-
 Gluconobacter oxydans 621H N-hydroxyaniline + 2 NADP+ + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 (2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide pH 8.0, 37°C Gluconobacter oxydans
0.12
-
 4-chloronitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.13
-
 4-nitrotoluene pH 8.0, 37°C Gluconobacter oxydans
0.15
-
 2-chloronitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.18
-
 nitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.18
-
 3-nitrotoluene pH 8.0, 37°C Gluconobacter oxydans
0.24
-
 1,3-dinitrobenzene pH 8.0, 37°C Gluconobacter oxydans
1.05
-
 NADPH pH 8.0, 37°C Gluconobacter oxydans
1.06
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide pH 8.0, 37°C Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.32
-
 2-chloronitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.33
-
 nitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.39
-
 4-chloronitrobenzene pH 8.0, 37°C Gluconobacter oxydans
0.46
-
 4-nitrotoluene pH 8.0, 37°C Gluconobacter oxydans
0.7
-
 3-nitrotoluene pH 8.0, 37°C Gluconobacter oxydans
1.9
-
 1,3-dinitrobenzene pH 8.0, 37°C Gluconobacter oxydans
6.9
-
 NADPH pH 8.0, 37°C Gluconobacter oxydans
20
-
 5-(aziridin-1-yl)-2,4-dinitrobenzamide pH 8.0, 37°C Gluconobacter oxydans
60
-
 (2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide pH 8.0, 37°C Gluconobacter oxydans