BRENDA - Enzyme Database
show all sequences of 1.7.1.16

Heterologous overexpression and biochemical characterization of a nitroreductase from Gluconobacter oxydans 621H

Yang, Y.; Lin, J.; Wei, D.; Mol. Biotechnol. 58, 428-440 (2016)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cd2+
1 mM, no residual activity
Gluconobacter oxydans
glutathione
1 mM, 41% residual activity
Gluconobacter oxydans
menadione
1 mM, 61% residual activity
Gluconobacter oxydans
Sodium azide
1 mM, 52% residual activity
Gluconobacter oxydans
Triton X-100
1% w/v, 84% residual activity
Gluconobacter oxydans
Tween 20
1% w/v, 64% residual activity
Gluconobacter oxydans
ZnSO4
1 mM, no residual activity
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide
pH 8.0, 37°C
Gluconobacter oxydans
0.054
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
pH 8.0, 37°C
Gluconobacter oxydans
0.128
-
1,3-dinitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.153
-
NADPH
pH 8.0, 37°C
Gluconobacter oxydans
0.264
-
3-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.282
-
4-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.309
-
4-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.468
-
2-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.545
-
nitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
Organism
Organism
UniProt
Commentary
Textmining
Gluconobacter oxydans
Q5FSN8
-
-
Gluconobacter oxydans 621H
Q5FSN8
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11000
-
substrate nitrobenzene, pH 8.0, 37°C
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+
i.e. anti-infective furacilin
740989
Gluconobacter oxydans
(2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
-
-
-
?
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+
i.e. anti-infective furacilin
740989
Gluconobacter oxydans 621H
(2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
-
-
-
?
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
? + 2 NADP+
-
-
-
?
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
? + 2 NADP+
-
-
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
3-nitrotoluene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
-
-
?
3-nitrotoluene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
4-nitrotoluene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
N-hydroxy-4-methylaniline + 2 NADP+ + H2O
-
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
? + 2 NADP+
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.1
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide
pH 8.0, 37°C
Gluconobacter oxydans
0.12
-
4-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.13
-
4-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.15
-
2-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.18
-
3-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.18
-
nitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.24
-
1,3-dinitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
1.05
-
NADPH
pH 8.0, 37°C
Gluconobacter oxydans
1.06
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
pH 8.0, 37°C
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cd2+
1 mM, no residual activity
Gluconobacter oxydans
glutathione
1 mM, 41% residual activity
Gluconobacter oxydans
menadione
1 mM, 61% residual activity
Gluconobacter oxydans
Sodium azide
1 mM, 52% residual activity
Gluconobacter oxydans
Triton X-100
1% w/v, 84% residual activity
Gluconobacter oxydans
Tween 20
1% w/v, 64% residual activity
Gluconobacter oxydans
ZnSO4
1 mM, no residual activity
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.016
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide
pH 8.0, 37°C
Gluconobacter oxydans
0.054
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
pH 8.0, 37°C
Gluconobacter oxydans
0.128
-
1,3-dinitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.153
-
NADPH
pH 8.0, 37°C
Gluconobacter oxydans
0.264
-
3-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.282
-
4-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.309
-
4-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.468
-
2-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.545
-
nitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11000
-
substrate nitrobenzene, pH 8.0, 37°C
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+
i.e. anti-infective furacilin
740989
Gluconobacter oxydans
(2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
-
-
-
?
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide + 2 NADPH + 2 H+
i.e. anti-infective furacilin
740989
Gluconobacter oxydans 621H
(2E)-2-[[5-(hydroxyamino)furan-2-yl]methylidene]hydrazine-1-carboxamide + 2 NADP+
-
-
-
?
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
? + 2 NADP+
-
-
-
?
1,3-dinitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
? + 2 NADP+
-
-
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
2-chloronitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
2-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
3-nitrotoluene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
-
-
?
3-nitrotoluene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
N-hydroxy-3-methylaniline + 2 NADP+ + H2O
-
-
-
?
4-chloronitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
4-chloro-N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
4-nitrotoluene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
N-hydroxy-4-methylaniline + 2 NADP+ + H2O
-
-
-
?
5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
? + 2 NADP+
enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740989
Gluconobacter oxydans 621H
N-hydroxyaniline + 2 NADP+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.1
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide
pH 8.0, 37°C
Gluconobacter oxydans
0.12
-
4-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.13
-
4-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.15
-
2-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.18
-
3-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.18
-
nitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.24
-
1,3-dinitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
1.05
-
NADPH
pH 8.0, 37°C
Gluconobacter oxydans
1.06
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
pH 8.0, 37°C
Gluconobacter oxydans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.32
-
2-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.33
-
nitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.39
-
4-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.46
-
4-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.7
-
3-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
1.9
-
1,3-dinitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
6.9
-
NADPH
pH 8.0, 37°C
Gluconobacter oxydans
20
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
pH 8.0, 37°C
Gluconobacter oxydans
60
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide
pH 8.0, 37°C
Gluconobacter oxydans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.32
-
2-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.33
-
nitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.39
-
4-chloronitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
0.46
-
4-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
0.7
-
3-nitrotoluene
pH 8.0, 37°C
Gluconobacter oxydans
1.9
-
1,3-dinitrobenzene
pH 8.0, 37°C
Gluconobacter oxydans
6.9
-
NADPH
pH 8.0, 37°C
Gluconobacter oxydans
20
-
5-(aziridin-1-yl)-2,4-dinitrobenzamide
pH 8.0, 37°C
Gluconobacter oxydans
60
-
(2E)-2-[(5-nitrofuran-2-yl)methylidene]hydrazine-1-carboxamide
pH 8.0, 37°C
Gluconobacter oxydans
Other publictions for EC 1.7.1.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740989
Yang
Heterologous overexpression an ...
Gluconobacter oxydans, Gluconobacter oxydans 621H
Mol. Biotechnol.
58
428-440
2016
-
-
-
-
-
-
7
9
-
-
-
-
-
2
-
-
-
-
-
-
1
-
13
-
-
-
-
-
9
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
9
-
-
-
-
-
-
-
-
-
-
1
-
13
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
740192
Li
In vivo imaging and detection ...
Danio rerio
Biosens. Bioelectron.
63
112-116
2015
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740325
Yuan
-
Isolation and characterization ...
Pseudomonas sp., Pseudomonas sp. N1
Chin. J. Appl. Environ. Biol.
19
1031-1034
2013
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741380
Isayev
In silico structure-function a ...
Enterobacter cloacae
Proteins
80
2728-2741
2012
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741093
Yanto
Nitroreductase from Salmonella ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Org. Biomol. Chem.
8
1826-1832
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
6
-
1
-
-
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
677659
Wu
Novel partial reductive pathwa ...
Comamonas sp., Comamonas sp. Ctestosteroni
Appl. Environ. Microbiol.
72
1759-1765
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
677735
Xiao
Characterization of genes invo ...
Pseudomonas putida, Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
166-171
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
14
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740133
Berne
Application of a microfluidic ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
Biomacromolecules
7
2631-2636
2006
-
1
1
-
-
-
-
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740929
Ho
-
Characterization of nitroreduc ...
Pseudomonas sp. HK-6
Korean J. Microbiol. Biotechnol.
32
230-237
2004
-
-
-
-
-
-
5
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
740596
Somerville
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
1
-
-
-
-
-
11
3
-
-
2
4
-
4
-
-
1
-
-
-
1
-
8
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
3
-
-
-
-
11
-
3
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739974
Rieble
Aromatic nitroreductase from t ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
2
-
7
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-