Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45

Somerville, C.C.; Nishino, S.F.; Spain, J.C.; J. Bacteriol. 177, 3837-3842 (1995)

Data extracted from this reference:

Activating Compound

Commentary

Organism

Structure

hydroxylamine

1 mM, 108% of initial activity

Pseudomonas pseudoalcaligenes

Inhibitors

Commentary

Organism

Structure

1-Naphthylamine

1 mM, 31% residual activity

Pseudomonas pseudoalcaligenes

CuSO4

0.5 mM, 78% residual activity

Pseudomonas pseudoalcaligenes

cysteine

1 mM, 87% residual activity

Pseudomonas pseudoalcaligenes

dicumarol

0.01 mM, 40% residual activity

Pseudomonas pseudoalcaligenes

N-ethylmaleimide

1 mM, 77% residual activity

Pseudomonas pseudoalcaligenes

neocuproin

1 mM, 39% residual activity

Pseudomonas pseudoalcaligenes

nitrite

1 mM, 77% residual activity

Pseudomonas pseudoalcaligenes

p-hydroxymercuribenzoate

1 mM, 46% residual activity

Pseudomonas pseudoalcaligenes

potassium cyanate

0.5 mM, 75% residual activity

Pseudomonas pseudoalcaligenes

salicyl hydroxamate

1 mM, 6% residual activity

Pseudomonas pseudoalcaligenes

Sodium azide

1 mM, 35% residual activity

Pseudomonas pseudoalcaligenes

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.005

-

nitrobenzene

temperature not specified in the publication, pH 8.0

Pseudomonas pseudoalcaligenes

0.009

-

menadione

temperature not specified in the publication, pH 8.0

Pseudomonas pseudoalcaligenes

0.183

-

NADPH

temperature not specified in the publication, pH 8.0

Pseudomonas pseudoalcaligenes

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

nitrobenzene + 2 NADPH + 2 H+

Pseudomonas pseudoalcaligenes

-

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

?

nitrobenzene + 2 NADPH + 2 H+

Pseudomonas pseudoalcaligenes JS45

-

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

?

nitrosobenzene + NADPH + H+

Pseudomonas pseudoalcaligenes

-

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

?

nitrosobenzene + NADPH + H+

Pseudomonas pseudoalcaligenes JS45

-

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

additional information

1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product

740596

Pseudomonas pseudoalcaligenes

?

-

-

-

-

additional information

1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product

740596

Pseudomonas pseudoalcaligenes JS45

?

-

-

-

-

nitrobenzene + 2 NADPH + 2 H+

-

740596

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

-

?

nitrobenzene + 2 NADPH + 2 H+

-

740596

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

-

?

nitrobenzene + menadione

-

740596

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + menadiol + H2O

-

-

-

?

nitrobenzene + menadione

-

740596

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + menadiol + H2O

-

-

-

?

nitrosobenzene + NADPH + H+

-

740596

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

-

?

nitrosobenzene + NADPH + H+

-

740596

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

-

?

Cofactor

Commentary

Organism

Structure

FMN

tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein

Pseudomonas pseudoalcaligenes

additional information

no cofactor: NADH

Pseudomonas pseudoalcaligenes

NADPH

-

Pseudomonas pseudoalcaligenes

Activating Compound

Commentary

Organism

Structure

hydroxylamine

1 mM, 108% of initial activity

Pseudomonas pseudoalcaligenes

Cofactor

Commentary

Organism

Structure

FMN

tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein

Pseudomonas pseudoalcaligenes

additional information

no cofactor: NADH

Pseudomonas pseudoalcaligenes

NADPH

-

Pseudomonas pseudoalcaligenes

Inhibitors

Commentary

Organism

Structure

1-Naphthylamine

1 mM, 31% residual activity

Pseudomonas pseudoalcaligenes

CuSO4

0.5 mM, 78% residual activity

Pseudomonas pseudoalcaligenes

cysteine

1 mM, 87% residual activity

Pseudomonas pseudoalcaligenes

dicumarol

0.01 mM, 40% residual activity

Pseudomonas pseudoalcaligenes

N-ethylmaleimide

1 mM, 77% residual activity

Pseudomonas pseudoalcaligenes

neocuproin

1 mM, 39% residual activity

Pseudomonas pseudoalcaligenes

nitrite

1 mM, 77% residual activity

Pseudomonas pseudoalcaligenes

p-hydroxymercuribenzoate

1 mM, 46% residual activity

Pseudomonas pseudoalcaligenes

potassium cyanate

0.5 mM, 75% residual activity

Pseudomonas pseudoalcaligenes

salicyl hydroxamate

1 mM, 6% residual activity

Pseudomonas pseudoalcaligenes

Sodium azide

1 mM, 35% residual activity

Pseudomonas pseudoalcaligenes

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.005

-

nitrobenzene

temperature not specified in the publication, pH 8.0

Pseudomonas pseudoalcaligenes

0.009

-

menadione

temperature not specified in the publication, pH 8.0

Pseudomonas pseudoalcaligenes

0.183

-

NADPH

temperature not specified in the publication, pH 8.0

Pseudomonas pseudoalcaligenes

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

nitrobenzene + 2 NADPH + 2 H+

Pseudomonas pseudoalcaligenes

-

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

?

nitrobenzene + 2 NADPH + 2 H+

Pseudomonas pseudoalcaligenes JS45

-

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

?

nitrosobenzene + NADPH + H+

Pseudomonas pseudoalcaligenes

-

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

?

nitrosobenzene + NADPH + H+

Pseudomonas pseudoalcaligenes JS45

-

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

additional information

1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product

740596

Pseudomonas pseudoalcaligenes

?

-

-

-

-

additional information

1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product

740596

Pseudomonas pseudoalcaligenes JS45

?

-

-

-

-

nitrobenzene + 2 NADPH + 2 H+

-

740596

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

-

?

nitrobenzene + 2 NADPH + 2 H+

-

740596

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + 2 NADP+ + H2O

N-hydroxyaniline is the only product detected

-

-

?

nitrobenzene + menadione

-

740596

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + menadiol + H2O

-

-

-

?

nitrobenzene + menadione

-

740596

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + menadiol + H2O

-

-

-

?

nitrosobenzene + NADPH + H+

-

740596

Pseudomonas pseudoalcaligenes

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

-

?

nitrosobenzene + NADPH + H+

-

740596

Pseudomonas pseudoalcaligenes JS45

N-hydroxyaniline + NADP+ + H2O

nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

740989

Yang

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428-440

2016

-

-

-

-

-

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7

9

-

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2

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1

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13

-

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9

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1

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1

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7

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9

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1

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13

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9

-

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9

9

740192

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In vivo imaging and detection ...

Danio rerio

Biosens. Bioelectron.

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112-116

2015

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1

1

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1

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1

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740325

Yuan

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Isolation and characterization ...

Pseudomonas sp., Pseudomonas sp. N1

Chin. J. Appl. Environ. Biol.

19

1031-1034

2013

-

1

1

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741380

Isayev

In silico structure-function a ...

Enterobacter cloacae

Proteins

80

2728-2741

2012

-

-

-

1

-

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-

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1

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741093

Yanto

Nitroreductase from Salmonella ...

Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2

Org. Biomol. Chem.

8

1826-1832

2010

-

-

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-

-

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-

2

-

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2

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6

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1

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3

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1

1

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2

-

6

-

-

-

3

-

1

1

-

-

-

-

-

-

-

-

677659

Wu

Novel partial reductive pathwa ...

Comamonas sp., Comamonas sp. Ctestosteroni

Appl. Environ. Microbiol.

72

1759-1765

2006

-

-

1

-

-

-

-

-

-

-

-

-

-

2

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2

-

1

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-

-

1

-

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-

1

1

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2

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1

1

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677735

Xiao

Characterization of genes invo ...

Pseudomonas putida, Pseudomonas putida ZWL73

Appl. Microbiol. Biotechnol.

73

166-171

2006

-

-

1

-

-

-

-

-

-

-

-

-

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7

-

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14

-

1

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2

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1

2

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-

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740133

Berne

Application of a microfluidic ...

Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45

Biomacromolecules

7

2631-2636

2006

-

1

1

-

-

-

-

6

-

-

-

-

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4

-

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1

-

-

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6

-

1

-

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1

1

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6

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1

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6

-

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-

-

-

-

-

-

-

740929

Ho

-

Characterization of nitroreduc ...

Pseudomonas sp. HK-6

Korean J. Microbiol. Biotechnol.

32

230-237

2004

-

-

-

-

-

-

5

-

-

-

1

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1

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1

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1

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2

1

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1

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1

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1

1

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1

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-

740596

Somerville

Purification and characterizat ...

Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45

J. Bacteriol.

177

3837-3842

1995

1

-

-

-

-

-

11

3

-

-

2

4

-

4

-

-

1

-

-

-

1

-

8

1

-

-

-

-

-

-

-

-

3

-

-

-

1

-

-

3

-

-

-

-

11

-

3

-

-

2

4

-

-

-

1

-

-

1

-

8

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

739974

Rieble

Aromatic nitroreductase from t ...

Phanerochaete chrysosporium

Biochem. Biophys. Res. Commun.

205

298-304

1994

-

-

-

-

-

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1

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1

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1

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2

-

7

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1

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1

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1

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1

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2

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7

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1

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1

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-