BRENDA - Enzyme Database
show all sequences of 1.7.1.16

Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45

Somerville, C.C.; Nishino, S.F.; Spain, J.C.; J. Bacteriol. 177, 3837-3842 (1995)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
hydroxylamine
1 mM, 108% of initial activity
Pseudomonas pseudoalcaligenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-Naphthylamine
1 mM, 31% residual activity
Pseudomonas pseudoalcaligenes
CuSO4
0.5 mM, 78% residual activity
Pseudomonas pseudoalcaligenes
cysteine
1 mM, 87% residual activity
Pseudomonas pseudoalcaligenes
dicumarol
0.01 mM, 40% residual activity
Pseudomonas pseudoalcaligenes
N-ethylmaleimide
1 mM, 77% residual activity
Pseudomonas pseudoalcaligenes
neocuproin
1 mM, 39% residual activity
Pseudomonas pseudoalcaligenes
nitrite
1 mM, 77% residual activity
Pseudomonas pseudoalcaligenes
p-hydroxymercuribenzoate
1 mM, 46% residual activity
Pseudomonas pseudoalcaligenes
potassium cyanate
0.5 mM, 75% residual activity
Pseudomonas pseudoalcaligenes
salicyl hydroxamate
1 mM, 6% residual activity
Pseudomonas pseudoalcaligenes
Sodium azide
1 mM, 35% residual activity
Pseudomonas pseudoalcaligenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
nitrobenzene
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
0.009
-
menadione
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
0.183
-
NADPH
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel fitration
Pseudomonas pseudoalcaligenes
33000
-
1 * 33000, SDS-PAGE
Pseudomonas pseudoalcaligenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
nitrobenzene + 2 NADPH + 2 H+
Pseudomonas pseudoalcaligenes
-
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
?
nitrobenzene + 2 NADPH + 2 H+
Pseudomonas pseudoalcaligenes JS45
-
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
?
nitrosobenzene + NADPH + H+
Pseudomonas pseudoalcaligenes
-
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
?
nitrosobenzene + NADPH + H+
Pseudomonas pseudoalcaligenes JS45
-
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas pseudoalcaligenes
Q6DLR9
-
-
Pseudomonas pseudoalcaligenes JS45
Q6DLR9
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas pseudoalcaligenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
245
-
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product
740596
Pseudomonas pseudoalcaligenes
?
-
-
-
-
additional information
1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product
740596
Pseudomonas pseudoalcaligenes JS45
?
-
-
-
-
nitrobenzene + 2 NADPH + 2 H+
-
740596
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740596
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
-
?
nitrobenzene + menadione
-
740596
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + menadiol + H2O
-
-
-
?
nitrobenzene + menadione
-
740596
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + menadiol + H2O
-
-
-
?
nitrosobenzene + NADPH + H+
-
740596
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
-
?
nitrosobenzene + NADPH + H+
-
740596
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Pseudomonas pseudoalcaligenes
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein
Pseudomonas pseudoalcaligenes
additional information
no cofactor: NADH
Pseudomonas pseudoalcaligenes
NADPH
-
Pseudomonas pseudoalcaligenes
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
hydroxylamine
1 mM, 108% of initial activity
Pseudomonas pseudoalcaligenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
tightly bound flavin mononucleotide cofactor in a ratio of 2 mol of flavin per mol of protein
Pseudomonas pseudoalcaligenes
additional information
no cofactor: NADH
Pseudomonas pseudoalcaligenes
NADPH
-
Pseudomonas pseudoalcaligenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-Naphthylamine
1 mM, 31% residual activity
Pseudomonas pseudoalcaligenes
CuSO4
0.5 mM, 78% residual activity
Pseudomonas pseudoalcaligenes
cysteine
1 mM, 87% residual activity
Pseudomonas pseudoalcaligenes
dicumarol
0.01 mM, 40% residual activity
Pseudomonas pseudoalcaligenes
N-ethylmaleimide
1 mM, 77% residual activity
Pseudomonas pseudoalcaligenes
neocuproin
1 mM, 39% residual activity
Pseudomonas pseudoalcaligenes
nitrite
1 mM, 77% residual activity
Pseudomonas pseudoalcaligenes
p-hydroxymercuribenzoate
1 mM, 46% residual activity
Pseudomonas pseudoalcaligenes
potassium cyanate
0.5 mM, 75% residual activity
Pseudomonas pseudoalcaligenes
salicyl hydroxamate
1 mM, 6% residual activity
Pseudomonas pseudoalcaligenes
Sodium azide
1 mM, 35% residual activity
Pseudomonas pseudoalcaligenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
nitrobenzene
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
0.009
-
menadione
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
0.183
-
NADPH
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
gel fitration
Pseudomonas pseudoalcaligenes
33000
-
1 * 33000, SDS-PAGE
Pseudomonas pseudoalcaligenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
nitrobenzene + 2 NADPH + 2 H+
Pseudomonas pseudoalcaligenes
-
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
?
nitrobenzene + 2 NADPH + 2 H+
Pseudomonas pseudoalcaligenes JS45
-
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
?
nitrosobenzene + NADPH + H+
Pseudomonas pseudoalcaligenes
-
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
?
nitrosobenzene + NADPH + H+
Pseudomonas pseudoalcaligenes JS45
-
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas pseudoalcaligenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
245
-
temperature not specified in the publication, pH 8.0
Pseudomonas pseudoalcaligenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product
740596
Pseudomonas pseudoalcaligenes
?
-
-
-
-
additional information
1.8 mol of NADPH is oxidized per mol of nitrobenzene converted to product
740596
Pseudomonas pseudoalcaligenes JS45
?
-
-
-
-
nitrobenzene + 2 NADPH + 2 H+
-
740596
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
-
?
nitrobenzene + 2 NADPH + 2 H+
-
740596
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + 2 NADP+ + H2O
N-hydroxyaniline is the only product detected
-
-
?
nitrobenzene + menadione
-
740596
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + menadiol + H2O
-
-
-
?
nitrobenzene + menadione
-
740596
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + menadiol + H2O
-
-
-
?
nitrosobenzene + NADPH + H+
-
740596
Pseudomonas pseudoalcaligenes
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
-
?
nitrosobenzene + NADPH + H+
-
740596
Pseudomonas pseudoalcaligenes JS45
N-hydroxyaniline + NADP+ + H2O
nitrosobenzene is not detected as an intermediate of nitrobenzene reduction, but nitrosobenzene is a substrate for the enzyme
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE
Pseudomonas pseudoalcaligenes
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740989
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7
9
-
-
-
-
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2
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-
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1
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13
-
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9
-
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1
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-
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-
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1
-
-
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7
-
9
-
-
-
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-
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1
-
13
-
-
-
-
9
-
-
-
-
-
-
-
-
9
9
740192
Li
In vivo imaging and detection ...
Danio rerio
Biosens. Bioelectron.
63
112-116
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1
1
-
-
-
-
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1
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1
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1
1
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1
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-
-
-
740325
Yuan
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Isolation and characterization ...
Pseudomonas sp., Pseudomonas sp. N1
Chin. J. Appl. Environ. Biol.
19
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-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
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-
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2
-
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-
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1
1
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-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741380
Isayev
In silico structure-function a ...
Enterobacter cloacae
Proteins
80
2728-2741
2012
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741093
Yanto
Nitroreductase from Salmonella ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Org. Biomol. Chem.
8
1826-1832
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
6
-
1
-
-
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
3
-
1
1
-
-
-
-
-
-
-
-
677659
Wu
Novel partial reductive pathwa ...
Comamonas sp., Comamonas sp. Ctestosteroni
Appl. Environ. Microbiol.
72
1759-1765
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
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-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
677735
Xiao
Characterization of genes invo ...
Pseudomonas putida, Pseudomonas putida ZWL73
Appl. Microbiol. Biotechnol.
73
166-171
2006
-
-
1
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
14
-
1
-
-
-
-
-
-
-
2
-
-
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-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740133
Berne
Application of a microfluidic ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
Biomacromolecules
7
2631-2636
2006
-
1
1
-
-
-
-
6
-
-
-
-
-
4
-
-
1
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740929
Ho
-
Characterization of nitroreduc ...
Pseudomonas sp. HK-6
Korean J. Microbiol. Biotechnol.
32
230-237
2004
-
-
-
-
-
-
5
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
2
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
-
1
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
740596
Somerville
Purification and characterizat ...
Pseudomonas pseudoalcaligenes, Pseudomonas pseudoalcaligenes JS45
J. Bacteriol.
177
3837-3842
1995
1
-
-
-
-
-
11
3
-
-
2
4
-
4
-
-
1
-
-
-
1
-
8
1
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
3
-
-
-
-
11
-
3
-
-
2
4
-
-
-
1
-
-
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739974
Rieble
Aromatic nitroreductase from t ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
205
298-304
1994
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
2
-
7
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
7
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-